AB192234

Anti-Nesprin1/Syne-1 抗体 [EPR14196]

Anti-Nesprin1/Syne-1 antibody [EPR14196]

RabMAb
Recombinant
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(20 Publications)

Rabbit Recombinant Monoclonal Nesprin1/Syne-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 20 publications.

別名を表示する

C6orf98, KIAA0796, KIAA1262, KIAA1756, MYNE1, SYNE1, Nesprin-1, Enaptin, KASH domain-containing protein 1, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1, KASH1, Myne-1, Syne-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

All lanes:

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HACAT cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 1011 kDa

false

Supplier Data

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

All lanes:

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

PC12 cell lysate at 10 µg

Lane 3:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 1011 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14196

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody is not suitable for using in tissue samples when testing in WB application.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin1 also known as Syne-1 is a large spectrin repeat protein with a mass of approximately 1002 kDa. It is part of the plakin family of proteins and plays an important role in linking the nuclear envelope to the actin cytoskeleton. Nesprin1 is widely expressed in skeletal and cardiac muscle tissues where it helps maintain nuclear positioning and structural integrity. This protein has several isoforms resulting from alternative splicing which allows it to perform diverse functions in different cellular contexts.

Biological function summary

Nesprin1 acts as an important component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. The LINC complex connects the nuclear envelope to the cytoskeleton facilitating mechanical signal transduction and cellular communication. Nesprin1's interactions with other elements of the cytoskeleton and nuclear envelope help regulate nuclear anchoring and positioning essential for cellular dynamics and function. In muscle cells Nesprin1 stabilizes the nucleus and synchronizes cellular responses to mechanical stress.

Pathways

Nesprin1 participates in nuclear-cytoskeletal coupling pathways that are vital for cellular biomechanics. It associates with proteins such as SUN1 and SUN2 within the LINC complex to mediate structural continuity between the nucleus and the cytoskeleton. Furthermore it plays a role in the mechanotransduction pathway linking external mechanical signals to gene expression changes. This connection is significant for maintaining cellular homeostasis and adaptability in response to mechanical stress.

Nesprin1 mutations are associated with muscular dystrophies particularly Emery-Dreifuss muscular dystrophy (EDMD). This condition involves muscle weakness and cardiac issues highlighting the importance of Nesprin1 in muscle tissue integrity. Additionally Nesprin1 has been linked to cardiomyopathies where its interaction with proteins like emerin and lamin A/C is important for maintaining cardiac muscle health. Alterations in these interactions can lead to disrupted nuclear architecture and contribute to the pathogenesis of these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenesis; a probable SUN3 : SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.

See full target information SYNE1

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 4:1501-1520 PubMed41073815

2025

Microtubule forces drive nuclear damage in LMNA cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Amiad Pavlov,Julie Heffler,Carmen Suay-Corredera,Mohammad Dehghany,Kaitlyn M Shen,Noam Zuela-Sopilniak,Rani Randell,Keita Uchida,Rajan Jain,Vivek Shenoy,Jan Lammerding,Benjamin Prosser

Cell death & disease 16:549 PubMed40695795

2025

ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhao Wang,Lu Zhu,Yubing Wang,Yue Han,Qingkun Wang,Wenqing Yang,Lei Zhao,Yu Zhang,Delong Pei,Wankun Huang,Aihua Jin,Zhenhua Lin,Junjie Piao

Frontiers in psychiatry 15:1396716 PubMed40276214

2025

Deficiency of autism susceptibility gene Trio in cerebellar Purkinje cells leads to delayed motor impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxin Wang,Yimeng Li,Dai Zhang,Wenzhi Sun,Jun Li

Scientific reports 15:6891 PubMed40011606

2025

High-intensity interval training alleviates STZ-induced muscle atrophy by restoration of nuclear positioning defects in C57BL/6 male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Masoud Rahmati,Rohollah Nikooie

EMBO reports 25:4728-4748 PubMed39317734

2024

SUN2 mediates calcium-triggered nuclear actin polymerization to cluster active RNA polymerase II.

Applications

Unspecified application

Species

Unspecified reactive species

Svenja Ulferts,Robert Grosse

NPJ microgravity 10:37 PubMed38521778

2024

Long non-coding RNA-NONMMMUT004552.2 regulates the unloading-induced bone loss through the miRNA-15b-5p/Syne1 in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhang,Yu Jing,Ang Zhang,JiShan Liu,Heming Yang,Xiaotong Lou,Liyan Xu,Min Liu,Yikun Zhang,Jianwen Gu

Oncology 102:868-879 PubMed38442705

2024

Nesprin1 Deficiency Is Associated with Poor Prognosis of Renal Cell Carcinoma and Resistance to Sunitinib Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Fukushima,Kohei Kobatake,Kento Miura,Kenshiro Takemoto,Ryoken Yamanaka,Ryo Tasaka,Yuki Kohada,Shunsuke Miyamoto,Yohei Sekino,Hiroyuki Kitano,Keisuke Goto,Kenichiro Ikeda,Akihiro Goriki,Keisuke Hieda,Osamu Kaminuma,Nobuyuki Hinata

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305867 PubMed38161226

2024

Coupling of Perinuclear Actin Cap and Nuclear Mechanics in Regulating Flow-Induced Yap Spatiotemporal Nucleocytoplasmic Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Tianxiang Ma,Xiao Liu,Haoran Su,Qiusheng Shi,Yuan He,Fan Wu,Chenxing Gao,Kexin Li,Zhuqing Liang,Dongrui Zhang,Xing Zhang,Ke Hu,Shangyu Li,Li Wang,Min Wang,Shuhua Yue,Weili Hong,Xun Chen,Jing Zhang,Lisha Zheng,Xiaoyan Deng,Pu Wang,Yubo Fan

Journal of translational medicine 21:690 PubMed37840136

2023

Systematic in vivo candidate evaluation uncovers therapeutic targets for LMNA dilated cardiomyopathy and risk of Lamin A toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Chia Yee Tan,Pui Shi Chan,Hansen Tan,Sung Wei Tan,Chang Jie Mick Lee,Jiong-Wei Wang,Shu Ye,Hendrikje Werner,Ying Jie Loh,Yin Loon Lee,Matthew Ackers-Johnson,Roger S Y Foo,Jianming Jiang

Cell reports 42:112534 PubMed37210724

2023

Activation of endoplasmic reticulum stress in premature aging via the inner nuclear membrane protein SUN2.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Vidak,Leonid A Serebryannyy,Gianluca Pegoraro,Tom Misteli

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Nesprin1/Syne-1 抗体 [EPR14196]

Anti-Nesprin1/Syne-1 antibody [EPR14196]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (20)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise