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リコンビナントRabMAb

Anti-Nephrin (phospho Y1217) 抗体 [EPTPG3] (ab80298)

Submit a review Submit a question References (8)
Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
  • Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
  • Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPTPG3] to Nephrin (phospho Y1217)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Nephrin (phospho Y1217) antibody [EPTPG3]
    Nephrin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPTPG3] to Nephrin (phospho Y1217)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blotmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: 293T transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors.

  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 20th November 2023.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPTPG3

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab80298の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
Dot blot
1/1000.
特記事項
WB
1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
Dot blot
1/1000.

ターゲット情報

  • 機能

    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.

  • 組織特異性

    Specifically expressed in podocytes of kidney glomeruli.

  • 関連疾患

    Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.

  • 配列類似性

    Belongs to the immunoglobulin superfamily.
    Contains 1 fibronectin type-III domain.
    Contains 8 Ig-like C2-type (immunoglobulin-like) domains.

  • 発生段階

    In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.

  • 翻訳後修飾

    Phosphorylated on tyrosine residues.

  • 細胞内局在

    Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CNF antibody
      • Nephrin antibody
      • Nephrosis 1 congenital Finnish type antibody
      • Nephrosis 1, congenital, Finnish type (nephrin) antibody
      • NPHN antibody
      • NPHN_HUMAN antibody
      • NPHS 1 antibody
      • Nphs1 antibody
      • Renal glomerulus specific cell adhesion receptor antibody
      • Renal glomerulus-specific cell adhesion receptor antibody
      see all

    画像

    • Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
      Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
      All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution

      Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged human Nephrin expression vector, whole cell lysate
      Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged Src expression vector, whole cell lysate
      Lane 4 : 293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 138 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
      Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
      All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution

      Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate
      Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate 15 µg. Then the membrane was incubated with Alkaline phosphatase

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 138 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
      Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)

      Dot blot analysis of Nephrin (pY1217) peptide and Nephrin non-phospho peptide labelling PKC alpha Nephrin (pY1217) with ab80298 at a 1/1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/20,000 dilution. Blocking and dilution buffer: 5% NFDM/TBST.

      Lane 1: Nephrin (pY1217) phospho peptide
      Lane 2: Nephrin non-phospho peptide

    参考文献 (8)

    ab80298 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80298 は 8 報の論文で使用されています。

    • Solanki AK  et al. Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair. J Biol Chem 297:101079 (2021). PubMed: 34391780
    • Tian Y  et al. Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress. Cell Death Dis 11:319 (2020). PubMed: 32371936
    • Yuan Q  et al. Role of pyruvate kinase M2-mediated metabolic reprogramming during podocyte differentiation. Cell Death Dis 11:355 (2020). PubMed: 32393782
    • New LA  et al. Nephrin Tyrosine Phosphorylation Is Required to Stabilize and Restore Podocyte Foot Process Architecture. J Am Soc Nephrol 27:2422-35 (2016). PubMed: 26802179
    • Kumagai T  et al. Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria. Am J Pathol 184:2211-24 (2014). PubMed: 24951831
    • Nezvitsky L  et al. Complement-mediated glomerular injury is reduced by inhibition of protein-tyrosine phosphatase 1B. Am J Physiol Renal Physiol 307:F634-47 (2014). PubMed: 25056348
    • Jeon J  et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells. J Biol Chem 287:28932-42 (2012). WB ; Human . PubMed: 22718751
    • Azhibekov TA  et al. TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. Am J Physiol Cell Physiol 301:C1351-9 (2011). PubMed: 21881001

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