Anti-NENF 抗体 (ab74474)
Key features and details
- Rabbit polyclonal to NENF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NENF antibody -
製品の詳細
Rabbit polyclonal to NENF -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).
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ポジティブ･コントロール
- Human kidney tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74474の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa). |
ターゲット情報
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機能
Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. -
配列類似性
Belongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain. -
ドメイン
The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 29937 Human
- Omim: 611874 Human
- SwissProt: Q9UMX5 Human
- Unigene: 461787 Human
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別名
- Cell growth inhibiting protein 47 antibody
- Cell immortalization-related protein 2 antibody
- CIR2 antibody
see all
画像
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Western blot - Anti-NENF antibody (ab74474)Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml
All lanes : Human kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 19 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74474 は 2 報の論文で使用されています。
- Tejedor G et al. MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis. Front Cell Dev Biol 9:579951 (2021). PubMed: 33738280
- Stefanska B et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . PubMed: 24763612