Anti-NEK9 (phospho T210) 抗体 (ab63553)
Key features and details
- Rabbit polyclonal to NEK9 (phospho T210)
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NEK9 (phospho T210) antibody
NEK9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NEK9 (phospho T210) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human NEK9 (phospho T210).
Database link: Q8TD19 -
ポジティブ･コントロール
- HeLa cells and extracts from HepG2 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63553の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 107 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 107 kDa). |
|
ICC/IF
1/500 - 1/1000. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. -
組織特異性
Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts. -
配列類似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Contains 6 RCC1 repeats. -
発生段階
Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells. -
ドメイン
Dimerizes through its coiled-coil domain. -
翻訳後修飾
Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 91754 Human
- Entrez Gene: 217718 Mouse
- Omim: 609798 Human
- SwissProt: Q8TD19 Human
- SwissProt: Q8K1R7 Mouse
- Unigene: 7200 Human
- Unigene: 29071 Mouse
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別名
- KIAA1995 antibody
- APUG antibody
- DKFZp434D0935 antibody
see all
画像
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Western blot - Anti-NEK9 (phospho T210) antibody (ab63553)All lanes : Anti-NEK9 (phospho T210) antibody (ab63553) at 1/500 dilution
Lane 1 : extracts from HepG2 cells at 30 µg/ml
Lane 2 : extracts from HepG2 cells at 30 µg with immunizing peptide at 10 µg
Predicted band size: 107 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-NEK9 (phospho T210) antibody (ab63553)ab63553 at 1/500 dilution staining HeLa cells, without (left) and with (right) immunizing peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK9 (phospho T210) antibody (ab63553)Ab63553 staining Human colon. Staining is localised to the cytoplasm and nucleus.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab63553 は 2 報の論文で使用されています。
- Kim M et al. Overexpression of the NEK9-EG5 axis is a novel metastatic marker in pathologic stage T3 colon cancer. Sci Rep 13:342 (2023). PubMed: 36611072
- Levinsohn JL et al. Somatic Mutations in NEK9 Cause Nevus Comedonicus. Am J Hum Genet 98:1030-1037 (2016). PubMed: 27153399