Anti-NEDD4 抗体 (ab117826)
Key features and details
- Rabbit polyclonal to NEDD4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NEDD4 antibody
NEDD4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NEDD4 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 725-775 of Human NEDD4 according to AAI36606.1
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ポジティブ･コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117826の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (1) |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
ドメイン
The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2. -
翻訳後修飾
Auto-ubiquitinated. -
細胞内局在
Cytoplasm. Cell membrane. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. - Information by UniProt
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参照データベース
- Entrez Gene: 4734 Human
- Omim: 602278 Human
- SwissProt: P46934 Human
- Unigene: 1565 Human
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別名
- Cell proliferation-inducing gene 53 protein antibody
- E3 ubiquitin protein ligase Nedd4 antibody
- E3 ubiquitin-protein ligase NEDD4 antibody
see all
画像
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Immunoprecipitation - Anti-NEDD4 antibody (ab117826)Detection of Human NEDD4 in HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab117826 at 6 µg/mg of lysate. Subsequent WB detection was done using another anti Human NEDD4 antibody. Detection: Chemiluminescence with exposure time of 30 seconds.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab117826 は論文での使用が確認できていません。