Anti-NEDD4-2 (phospho S448) 抗体 [EPR8269(2)] (ab168349)
![Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)](https://www.abcam.co.jp/ps/products/168/ab168349/Images/ab168349-168675-anti-nedd4-2-phospho-s448-antibody-epr82692-immunoprecipitation.jpg "Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)]
NEDD4-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human NEDD4-2 (phospho S448). Corresponding to residues surrounding Serine 448.
Database link: Q96PU5 -
ポジティブ･コントロール
- 293T cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8269(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 110-135 kDa (predicted molecular weight: 112 kDa).
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 110-135 kDa (predicted molecular weight: 112 kDa). |
|
IP
1/10 - 1/100. |
追加情報
Is unsuitable for ICC/IF or IHC-P.
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. -
組織特異性
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
翻訳後修飾
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. -
細胞内局在
Cytoplasm. May be recruited to exosomes by NDFIP1. - Information by UniProt
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参照データベース
- Entrez Gene: 23327 Human
- Entrez Gene: 83814 Mouse
- Entrez Gene: 291553 Rat
- Omim: 606384 Human
- SwissProt: Q96PU5 Human
- SwissProt: Q8CFI0 Mouse
- Unigene: 185677 Human
- Unigene: 98668 Mouse
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別名
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- E3 ubiquitin-protein ligase NEDD4-like antibody
- KIAA0439 antibody
see all
画像
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Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)](https://www.abcam.co.jp/ps/products/168/ab168349/Images/ab168349-168672-anti-nedd4-2-phospho-s448-antibody-epr82692-western-blot.jpg)
Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa
Additional bands at: 110-135 kDa. We are unsure as to the identity of these extra bands. -
![Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)](https://www.abcam.co.jp/ps/products/168/ab168349/Images/ab168349-3-benefits-of-recombinant-antibodies.png)
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab168349 は 10 報の論文で使用されています。
- Zhang H et al. Pharmacological suppression of Nedd4-2 rescues the reduction of Kv11.1 channels in pathological cardiac hypertrophy. Front Pharmacol 13:942769 (2022). PubMed: 36059970
- Tang LL et al. Aldosterone-stimulated endothelial epithelial sodium channel (EnNaC) plays a role in cold exposure-induced hypertension in rats. Front Pharmacol 13:970812 (2022). PubMed: 36278222
- Kim JE et al. AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats. Biomedicines 9:N/A (2021). PubMed: 34440273
- Wang QS et al. NaHS or Lovastatin Attenuates Cyclosporine A-Induced Hypertension in Rats by Inhibiting Epithelial Sodium Channels. Front Pharmacol 12:665111 (2021). PubMed: 34122084
- Liang C et al. Homocysteine Causes Endothelial Dysfunction via Inflammatory Factor-Mediated Activation of Epithelial Sodium Channel (ENaC). Front Cell Dev Biol 9:672335 (2021). PubMed: 34222246
- Yang X et al. Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats. Oxid Med Cell Longev 2020:3921897 (2020). PubMed: 33194000
- Lü L et al. JAK3 restrains inflammatory responses and protects against periodontal disease through Wnt3a signaling. FASEB J 34:9120-9140 (2020). PubMed: 32433819
- Wu MM et al. Lovastatin attenuates hypertension induced by renal tubule-specific knockout of ATP-binding cassette transporter A1, by inhibiting epithelial sodium channels. Br J Pharmacol 176:3695-3711 (2019). PubMed: 31222723
- Kemp BA et al. Ghrelin-Induced Sodium Reabsorption Is Mediated by PKA and Microtubule-Dependent aENaC Translocation in Female Rats. J Endocr Soc 3:2088-2106 (2019). PubMed: 31663064
- Salker MS et al. LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1. Cell Physiol Biochem 39:1295-306 (2016). PubMed: 27606670