Anti-NEDD4-2 抗体 (ab46521)
Key features and details
- Rabbit polyclonal to NEDD4-2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NEDD4-2 antibody
NEDD4-2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NEDD4-2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46521の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/1000.
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| ICC/IF |
1/200.
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| WB | (3) |
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
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| 特記事項 |
|---|
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IHC-P
1/1000. |
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ICC/IF
1/200. |
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WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. -
組織特異性
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
翻訳後修飾
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. -
細胞内局在
Cytoplasm. May be recruited to exosomes by NDFIP1. - Information by UniProt
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参照データベース
- Entrez Gene: 23327 Human
- Omim: 606384 Human
- SwissProt: Q96PU5 Human
- Unigene: 185677 Human
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別名
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- E3 ubiquitin-protein ligase NEDD4-like antibody
- KIAA0439 antibody
see all
画像
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Western blot - Anti-NEDD4-2 antibody (ab46521)All lanes : Anti-NEDD4-2 antibody (ab46521)
Lane 1 : Extract from non-transfected HEK293 cells
Lane 2 : Extract from HEK293 cells transfected with NEDD4-2
Predicted band size: 120 kDa
Observed band size: 120 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD4-2 antibody (ab46521)ab46251 (1/1000) staining NEDD4-2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and membrane staining of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-NEDD4-2 antibody (ab46521)ICC/IF image of ab46521 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with ab46521 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (30)
ab46521 は 30 報の論文で使用されています。
- Sun YF et al. Involvement of miR-495 overexpression in the pathogenesis of bronchopulmonary dysplasia in preterm infants via the targeting of NEDD4L-ENaC pathway. Ann Transl Med 11:4 (2023). PubMed: 36760249
- Li G et al. Downregulation of NEDD4L by EGFR signaling promotes the development of lung adenocarcinoma. J Transl Med 20:47 (2022). PubMed: 35090513
- Deftu AF et al. The Antidiabetic Drug Metformin Regulates Voltage-Gated Sodium Channel NaV1.7 via the Ubiquitin-Ligase NEDD4-2. eNeuro 9:N/A (2022). PubMed: 35131865
- Wang C et al. The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells. Anim Nutr 10:12-18 (2022). PubMed: 35601254
- Saha B et al. WNK1 is a chloride-stimulated scaffold that regulates mTORC2 activity and ion transport. J Cell Sci 135:N/A (2022). PubMed: 36373794
