Anti-Nectin 2 抗体 [B-C12] - BSA and Azide free (ab47171)
![Flow Cytometry - Anti-Nectin 2 antibody [B-C12] (ab47171)](https://www.abcam.co.jp/ps/products/47/ab47171/Images/ab47171_FCS.jpg "Flow Cytometry - Anti-Nectin 2 antibody [B-C12] (ab47171)")
Key features and details
- Mouse monoclonal [B-C12] to Nectin 2 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Nectin 2 antibody [B-C12] - BSA and Azide free
Nectin 2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-C12] to Nectin 2 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Nectin 2. KG-1 cell line
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ポジティブ･コントロール
- KG-1 cell line
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特記事項
This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
B-C12 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47171の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 10 µg/ml.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 - 10 µg/ml. |
|
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Probable cell adhesion protein. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5819 Human
- Omim: 600798 Human
- SwissProt: Q92692 Human
- Unigene: 655455 Human
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別名
- CD 112 antibody
- CD112 antibody
- CD112 antigen antibody
see all
画像
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Flow Cytometry - Anti-Nectin 2 antibody [B-C12] (ab47171)
A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nectin 2 antibody [B-C12] (ab47171)](https://www.abcam.co.jp/ps/products/47/ab47171/Images/Nectin-2-Primary-antibodies-ab47171-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nectin 2 antibody [B-C12] (ab47171)ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab47171 は 2 報の論文で使用されています。
- García-Cuesta EM et al. NKG2D is a Key Receptor for Recognition of Bladder Cancer Cells by IL-2-Activated NK Cells and BCG Promotes NK Cell Activation. Front Immunol 6:284 (2015). PubMed: 26106390
- Ogbomo H et al. Myxoma virus infection promotes NK lysis of malignant gliomas in vitro and in vivo. PLoS One 8:e66825 (2013). Flow Cyt ; Human . PubMed: 23762498
