Anti-NEAS 抗体 [D8B7]

• ICC

Supplier Data

Immunocytochemistry - Anti-NEAS antibody [D8B7] (AB11755)

ICC staining of purified ab11755 on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 μg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 μg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar : 50 μm

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [D8B7] (AB11755)

IHC staining of purified ab11755 on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 μg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar : 50 μm

• ICC

Supplier Data

Immunocytochemistry - Anti-NEAS antibody [D8B7] (AB11755)

ICC staining of purified ab11755 on 3T3 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 μg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 μg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar : 50 μm

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [D8B7] (AB11755)

IHC staining of purified ab11755 on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 μg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar : 50 μm

• IHC-P

AbReview8077****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [D8B7] (AB11755)

ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

This image was generated using the ascites version of the product.

This image is courtesy of an anonymous Abreview

• WB

AbReview8523****

Western blot - Anti-NEAS antibody [D8B7] (AB11755)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-NEAS antibody [D8B7] (ab11755) at 1/1000 dilution

All lanes:

3T3 whole cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated goat anti-mouse at 1/5000 dilution

Predicted band size: 285 kDa

Observed band size: 250 kDa

false

Exposure time: 3min

This image is courtesy of an anonymous Abreview

• ICC

Unknown

Immunocytochemistry - Anti-NEAS antibody [D8B7] (AB11755)

IF using ab11755.

This image was generated using the ascites version of the product.

• WB

Supplier Data

Western blot - Anti-NEAS antibody [D8B7] (AB11755)

Lane 1:

Western blot - Anti-NEAS antibody [D8B7] (ab11755)

Lanes 2 - 3:

Western blot - Anti-NEAS antibody [D8B7] (ab11755) at 0.1 µg/mL

Lane 2:

Drosophila head lysate at 20 µg

Lane 3:

Drosophila S2 (embryonic) cell lysate at 20 µg

Secondary

All lanes:

HRP-labeled goat anti-mouse IgG

Predicted band size: 285 kDa

false