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リコンビナントRabMAb

Anti-NDUFS8 抗体 [EPR11892] (ab170936)

Reviews (1) Submit a question References (3)
Western blot - Anti-NDUFS8 antibody [EPR11892] (ab170936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS8 antibody [EPR11892] (ab170936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS8 antibody [EPR11892] (ab170936)
  • Flow Cytometry (Intracellular) - Anti-NDUFS8 antibody [EPR11892] (ab170936)
  • Immunoprecipitation - Anti-NDUFS8 antibody [EPR11892] (ab170936)
  • Anti-NDUFS8 antibody [EPR11892] (ab170936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11892] to NDUFS8
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NDUFS8 antibody [EPR11892]
    NDUFS8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11892] to NDUFS8

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human NDUFS8 aa 1-100. The exact sequence is proprietary.
    Database link: O00217

  • ポジティブ･コントロール

    • HeLa, HepG2 and Jurkat cell lysates, Human liver tissue, Human thyroid carcinoma tissue, permeabilized Jurkat cells, immunoprecipitation pellet from HeLa cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab170936の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.

ターゲット情報

  • 機能

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.

  • 関連疾患

    Defects in NDUFS8 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.

  • 配列類似性

    Belongs to the complex I 23 kDa subunit family.
    Contains 2 4Fe-4S ferredoxin-type domains.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CI 23kD antibody
      • CI-23kD antibody
      • Complex I 23kD antibody
      • Complex I-23kD antibody
      • EC 1.6.5.3 antibody
      • EC 1.6.99.3 antibody
      • Human mitochondrial NADH dehydrogenase ubiquinone Fe S protein 8 antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial antibody
      • NADH ubiquinone oxidoreductase 23 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 23 kDa subunit antibody
      • NDUFS8 antibody
      • NDUS8_HUMAN antibody
      • TYKY antibody
      • TYKY subunit antibody
      • Ubiquinoneiron sulfur protein 8, mitochondrial precursor antibody
      see all

    画像

    • Western blot - Anti-NDUFS8 antibody [EPR11892] (ab170936)
      Western blot - Anti-NDUFS8 antibody [EPR11892] (ab170936)
      All lanes : Anti-NDUFS8 antibody [EPR11892] (ab170936) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS8 antibody [EPR11892] (ab170936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS8 antibody [EPR11892] (ab170936)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NDUFS8 with ab170936 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS8 antibody [EPR11892] (ab170936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS8 antibody [EPR11892] (ab170936)

      Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling NDUFS8 with ab170936 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-NDUFS8 antibody [EPR11892] (ab170936)
      Flow Cytometry (Intracellular) - Anti-NDUFS8 antibody [EPR11892] (ab170936)

      Intracellular flow cytometric analysis of permeabilized Jurkat cells usingab170936 (red)at 1/10 dilutionor a rabbit IgG (negative) (green). 

       

       

    • Immunoprecipitation - Anti-NDUFS8 antibody [EPR11892] (ab170936)
      Immunoprecipitation - Anti-NDUFS8 antibody [EPR11892] (ab170936)

      Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1X PBS (negative control) using ab170936 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-NDUFS8 antibody [EPR11892] (ab170936)
      Anti-NDUFS8 antibody [EPR11892] (ab170936)

    参考文献 (3)

    ab170936 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab170936 は 3 報の論文で使用されています。

    • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
    • Bezawork-Geleta A  et al. Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints. Nat Commun 9:2221 (2018). PubMed: 29880867
    • Maio N  et al. A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell Metab 25:945-953.e6 (2017). PubMed: 28380382

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NDUFS8 antibody [EPR11892]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Neutral buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 May 17 2019

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