AB241346

Anti-NDUFS8 抗体

Name: Anti-NDUFS8 抗体 (ab241346) | Abcam
Brand: Abcam Limited
SKU: AB241346
Price: 75000 JPY
Availability: InStock

Anti-NDUFS8 antibody

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal NDUFS8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus.

別名を表示する

Complex I-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, CI-23kD, NDUFS8

2 Images

Supplier Data

Immunoprecipitation - Anti-NDUFS8 antibody (AB241346)

NDUFS8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241346 used for IP at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241346 at 1 μg/ml.

Lane 1 : ab241346 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NDUFS8 antibody (ab241346)

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-NDUFS8 antibody (AB241346)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-NDUFS8 antibody (ab241346) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 23 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00217

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab241346 was affinity purified using an epitope specific to NDUFS8 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The NDUFS8 protein also known as NADH:ubiquinone oxidoreductase core subunit S8 serves an important function in the mitochondrial electron transport chain. It weighs approximately 23 kDa and is expressed in many tissues due to its essential role in energy production. NDUFS8 is a part of the larger mitochondrial complex I where it plays a role in transferring electrons derived from NADH to ubiquinone. Through this process NDUFS8 contributes to the creation of a proton gradient used for ATP synthesis.

Biological function summary

The NDUFS8 protein functions as an integral component of the mitochondrial respiratory complex I. Complex I is essential for cellular respiration and contributes to the production of ATP which cells use as an energy source. NDUFS8 helps facilitate the electron transport chain as a subunit of this multi-protein complex ensuring effective electron transfer and energy conversion within the mitochondria.

Pathways

The NDUFS8 protein operates within the oxidative phosphorylation pathway a major energy-producing pathway in cells. It also has connections with the tricarboxylic acid (TCA) cycle where NADH is generated and enters the electron transport chain through NDUFS8. The NDUFS8 protein collaborates with other subunits of complex I such as NDUFV1 and NDUFV2 to ensure proper electron flow and energy production.

Mutations in the NDUFS8 protein can lead to mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy. These conditions are characterized by severe energy production deficiencies due to impaired function of complex I. NDUFS8 mutations can interact with other proteins implicated in these disorders including NDUFAF2 which is involved in the assembly of complex I further disrupting normal mitochondrial activity and energy metabolism.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 22499348). Essential for the catalytic activity and assembly of complex I (PubMed : 22499348).

See full target information NDUFS8

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 39:596-604 PubMed36912285

2023

Tan IIA mitigates vascular smooth muscle cell proliferation and migration induced by ox-LDL through the miR-137/TRPC3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Zhi Gao,Qing-Long Guan

Brain communications 4:fcac102 PubMed35602653

2022

Disruption of cellular iron homeostasis by missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Russell P Saneto,Richard Steet,Marcio A Sotero de Menezes,Cindy Skinner,Tracey A Rouault

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFS8 抗体

Anti-NDUFS8 antibody

Immunoprecipitation - Anti-NDUFS8 antibody (AB241346)

Western blot - Anti-NDUFS8 antibody (AB241346)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise