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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-NDUFS3 抗体 [EPR12781] (ab183733)

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Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [EPR12781] (ab183733)
  • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
  • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
  • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
  • Anti-NDUFS3 antibody [EPR12781] (ab183733)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12781] to NDUFS3
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NDUFS3 antibody [EPR12781]
    NDUFS3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12781] to NDUFS3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal heart, Human fetal brain, HepG2, HeLa, HEK-293T, HL-60, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates; Human hepatocellular carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183733の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • 配列類似性

    Belongs to the complex I 30 kDa subunit family.

  • 細胞内局在

    Mitochondrion inner membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CI 30 antibody
      • CI 30KD antibody
      • CI-30kD antibody
      • Complex I 30KD antibody
      • Complex I 30kDa subunit antibody
      • COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody
      • Complex I-30kD antibody
      • mitochondrial antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody
      • NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody
      • NADH dehydrogenase ubiquinone 30 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 30 kDa subunit antibody
      • NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody
      • NDUFS3 antibody
      • NDUS3_HUMAN antibody
      see all

    画像

    • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      All lanes : Anti-NDUFS3 antibody [EPR12781] (ab183733) at 1/10000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : NDUFS3 knockout HEK-293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 30 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab183733 observed at 27 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

      ab183733 was shown to react with NDUFS3 in HEK-293T wild-type cells in western blot with loss of signal observed in NDUFS3 knockout cell line ab266419 (NDUFS3 knockout cell lysate ab257556). Wild-type and NDUFS3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab183733 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [EPR12781] (ab183733)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NDUFS3 with ab183733 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      All lanes : Anti-NDUFS3 antibody [EPR12781] (ab183733) at 1/10000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : NDUFS3 knockout HEK-293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HL60 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 30 kDa
      Observed band size: 30 kDa



      Lanes 1- 4: Merged signal (red and green). Green - ab183733 observed at 30 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

       ab183733 was shown to react with NDUFS3 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266419 (knockout cell lysate ab257556) was used. Wild-type HEK-293T and NDUFS3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab183733 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      All lanes : Anti-NDUFS3 antibody [EPR12781] (ab183733) at 1/100000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 26 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      Western blot - Anti-NDUFS3 antibody [EPR12781] (ab183733)
      All lanes : Anti-NDUFS3 antibody [EPR12781] (ab183733) at 1/20000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 26 kDa why is the actual band size different from the predicted?

    • Anti-NDUFS3 antibody [EPR12781] (ab183733)
      Anti-NDUFS3 antibody [EPR12781] (ab183733)

    参考文献 (0)

    ab183733 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183733 は論文での使用が確認できていません。

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