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リコンビナントRabMAb

Anti-Ndufs1 抗体 [EPR11521(B)] (ab169540)

Reviews (2) Submit a question References (23)
Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
  • Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11521(B)] to Ndufs1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Ndufs1 antibody [EPR11521(B)]
    Ndufs1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11521(B)] to Ndufs1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Raji, HepG2, HeLa and Jurkat cell lysates. Human cardiac muscle and Human liver tissue. HepG2 and permeabilized Jurkat cells.

  • 特記事項

    Ndufs1 is a protein encoded by the NDUFS1 gene. The NDUFS1 protein is the largest subunit of complex 1 which is located on the inner mitochondrial membrane. This complex is critical to the oxidative phosphorylation which occurs within the mitochondria.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab169540の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB (2)
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.

ターゲット情報

  • 機能

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

  • 関連疾患

    Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.

  • 配列類似性

    Belongs to the complex I 75 kDa subunit family.
    Contains 1 2Fe-2S ferredoxin-type domain.

  • 細胞内局在

    Mitochondrion inner membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CI-75kD antibody
    • Complex I 75Kd antibody
    • Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
    • Complex I-75kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
    • NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
    • NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
    • NDUFS1 antibody
    • NDUS1_HUMAN antibody
    • PRO1304 antibody
    see all

画像

  • Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 7 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) without mitochondria fraction cell lysate
    Lane 2 : HepG2 mitochondria fraction cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling Ndufs1 with ab169540 at 1/50 dilution (2.6 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

     

    Confocal image showing cytoplasmic staining in Raji cell line.

     

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

     

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Ndufs1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate with AB169540 at 1/20 dilution (0.6ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB169540 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

    Lane 2: AB169540 IP in Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB169540 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Ndufs1 with ab169540 at 1/100 (1.29 ug/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Positive staining on human kidney. The section was incubated with ab169540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Ndufs1 with ab169540 at 1/100 (1.29 ug/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Positive staining on rat kidney. The section was incubated with ab169540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Ndufs1 with ab169540 at 1/100 (1.29 ug/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Positive staining on mouse kidney. The section was incubated with ab169540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 min.

  • Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling Ndufs1 with ab169540 at 1/20 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

参考文献 (23)

ab169540 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab169540 は 23 報の論文で使用されています。

  • Ledahawsky LM  et al. The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathways in vivo. FEBS J 289:3894-3914 (2022). PubMed: 35092170
  • Contreras L  et al. Broad Transcriptomic Impact of Sorafenib and Its Relation to the Antitumoral Properties in Liver Cancer Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35267509
  • Wu Y  et al. Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks. J Anim Sci Biotechnol 13:61 (2022). PubMed: 35581591
  • Jin X  et al. Glycyrrhetinic acid restricts mitochondrial energy metabolism by targeting SHMT2. iScience 25:104349 (2022). PubMed: 35602963
  • Tong WH  et al. Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia. Heliyon 8:e10371 (2022). PubMed: 36061025
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Ndufs1 antibody [EPR11521(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 20 2020

Western blot abreview for Anti-Ndufs1 antibody [EPR11521(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (colon cancer)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
colon cancer
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 09 2020

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