Anti-Ndufs1 抗体 (ab185733)
Key features and details
- Rabbit polyclonal to Ndufs1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Ndufs1 antibody
Ndufs1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ndufs1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan
-
免疫原
Recombinant fragment corresponding to Human Ndufs1 aa 50-300.
Database link: P28331 -
ポジティブ･コントロール
- HeLa, Raji, H460, PC3, 293T, HepG2, Mouse kidney, Mouse heart and Mouse intestine tissue extracts.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185733の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 79 kDa.
|
|
| IHC-P |
1/50 - 1/200.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 79 kDa. |
|
IHC-P
1/50 - 1/200. |
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. -
関連疾患
Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
配列類似性
Belongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 288380 Cow
- Entrez Gene: 4719 Human
- Entrez Gene: 227197 Mouse
- Entrez Gene: 301458 Rat
- Omim: 157655 Human
- SwissProt: Q0MQG2 Chimpanzee
- SwissProt: P15690 Cow
- SwissProt: Q4R6K9 Cynomolgus monkey
see all -
別名
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
see all
画像
-
Western blot - Anti-Ndufs1 antibody (ab185733)All lanes : Anti-Ndufs1 antibody (ab185733) at 1/500 dilution
Lane 1 : HeLa cell line extract
Lane 2 : Raji cell line extract
Lane 3 : H460 cell line extract
Lane 4 : PC3 cell line extract
Lane 5 : 293T cell line extract
Lane 6 : HepG2 cell line extract
Lane 7 : Mouse kidney tissue extract
Lane 8 : Mouse heart tissue extract
Lane 9 : Mouse intestine tissue extract
Predicted band size: 79 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (ab185733)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Ndufs1 with ab185733 at 1/200. Magnification: 400x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (ab185733)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Ndufs1 with ab185733 at 1/200. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody (ab185733)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185733. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab185733 は 1 報の論文で使用されています。
- Maio N et al. Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures. Brain Commun 4:fcac102 (2022). PubMed: 35602653