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AB192243

Anti-NDUFB4 抗体 [EPR16240]

Anti-NDUFB4 antibody [EPR16240]

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(2 Publications)

Rabbit Recombinant Monoclonal NDUFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications.

別名を表示する

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, NADH-ubiquinone oxidoreductase B15 subunit, CI-B15, NDUFB4

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] (AB192243)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] (AB192243)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized A549 cells labeling NDUFB4 with ab192243 at 1/50 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

The two negative controls are anti-NDUFB4 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (AB192243)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (AB192243)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (AB192243)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (AB192243)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-NDUFB4 antibody [EPR16240] (AB192243)
  • WB

Supplier Data

Western blot - Anti-NDUFB4 antibody [EPR16240] (AB192243)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/20000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

Western blot - Anti-NDUFB4 antibody [EPR16240] (AB192243)
  • WB

Supplier Data

Western blot - Anti-NDUFB4 antibody [EPR16240] (AB192243)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

Western blot - Anti-NDUFB4 antibody [EPR16240] (AB192243)
  • WB

Supplier Data

Western blot - Anti-NDUFB4 antibody [EPR16240] (AB192243)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/5000 dilution

All lanes:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16240

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB110243

Anti-NDUFB4 antibody [17G3D9E12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)

  • 5
  • 1

組合せ製品

Primary Antibodies

AB200198

Anti-NDUFB9 antibody [EPR15955-78]

RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (AB200198)

  • 0
  • 0
Primary Antibodies

AB202554

Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control

RabMAb|Recombinant|Lab Essentials|Advanced Validation|20ul selling size

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

  • 5
  • 2
Primary Antibodies

AB192878

Anti-NDUFB8 antibody [EPR15961]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (AB192878)

  • 5
  • 1
Primary Antibodies

AB203832

Anti-UQCRC2 antibody [EPR13051]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (AB203832)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB4 also known as NADH: ubiquinone oxidoreductase subunit B4 plays a role in the mitochondrial respiratory chain complex I. Weighing approximately 15 kDa NDUFB4 is expressed in mitochondria across various cell types. It couples electron transfer from NADH to ubiquinone thereby contributing to energy production process in cells. NDUFB4 is important in maintaining mitochondrial functionality due to its role in the electron transport chain.
Biological function summary

NADH: ubiquinone oxidoreductase subunit B4 is part of the NADH dehydrogenase complex I. This complex is involved in the initial step of the mitochondrial electron transport chain. NDUFB4 supports the bioenergetic functions of cells by facilitating redox reactions. It also helps establish the electrochemical gradient needed for ATP synthesis. This protein interacts synergistically with other subunits in complex I to ensure efficient energy conversion.

Pathways

NADH dehydrogenase complex I which includes NDUFB4 is central in oxidative phosphorylation. This process supports energy production by converting NADH and FADH2 into ATP. NDUFB4 is integrally linked with proteins like NDUFS2 and NDUFV1 within this pathway ensuring electron transport is seamless. Complex I contributes to metabolic processes like the TCA cycle by feeding the mitochondria with necessary energy intermediates.

Malfunction of NDUFB4 in complex I links to mitochondrial diseases such as Leigh syndrome. This syndrome affects central nervous system development. Mitochondrial encephalopathies are another group where NDUFB4 involvement is observed. In these conditions proteins like NDUFA1 and mitochondrial DNA mutations can manifest similar neurodegenerative symptoms due to impaired oxidative phosphorylation. Therefore understanding NDUFB4’s function helps in identifying therapeutic interventions for these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFB4
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 16: PubMed37497665

2023

OPA1 deficiency impairs oxidative metabolism in cycling cells, underlining a translational approach for degenerative diseases.

Applications

WB

Species

Human

Aurélie M C Millet,Corentin Coustham,Camille Champigny,Marlène Botella,Christine Demeilliers,Anne Devin,Anne Galinier,Pascale Belenguer,Joel Bordeneuve-Guibé,Noélie Davezac

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:3331-3343 PubMed33707295

2021

Mutations Cause Mechanotransduction Defects in Cochlear Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alix Trouillet,Katharine K Miller,Shefin Sam George,Pei Wang,Noor-E-Seher Ali,Anthony Ricci,Nicolas Grillet
View all publications
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Abcam product promise

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