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リコンビナントRabMAb

Anti-NDUFB2 抗体 [EPR15569] (ab186748)

Submit a review Submit a question References (1)
Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (ab186748)
  • Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] (ab186748)
  • Anti-NDUFB2 antibody [EPR15569] (ab186748)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15569] to NDUFB2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NDUFB2 antibody [EPR15569]
    NDUFB2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15569] to NDUFB2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human skeletal muscle, fetal heart, fetal brain, fetal kidney, HeLa and 293 lysates; Rat kidney, heart and brain lysates; Mouse heart, kidney and spleen lysates; Human endometrium tissue; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186748の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • 配列類似性

    Belongs to the complex I NDUFB2 subunit family.

  • 細胞内局在

    Mitochondrion inner membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CI-AGGG antibody
      • Complex I-AGGG antibody
      • mitochondrial antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 antibody
      • NADH-ubiquinone oxidoreductase AGGG subunit antibody
      • NDUB2_HUMAN antibody
      • NDUFB2 antibody
      see all

    画像

    • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/10000 dilution + Human fetal heart lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 12 kDa

    • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 12 kDa

    • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

      Lane 1 : Human skeletal muscle tissue lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 12 kDa

    • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/2000 dilution

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Mouse spleen tissue lysate
      Lane 4 : Rat brain tissue lysate
      Lane 5 : Rat heart tissue lysate
      Lane 6 : Rat kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 12 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (ab186748)

      Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFB2 with ab186748 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] (ab186748)

      Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling NDUFB2 with ab186748 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Anti-NDUFB2 antibody [EPR15569] (ab186748)
      Anti-NDUFB2 antibody [EPR15569] (ab186748)

    参考文献 (1)

    ab186748 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186748 は 1 報の論文で使用されています。

    • Liang JR  et al. A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Cell 180:1160-1177.e20 (2020). PubMed: 32160526

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