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リコンビナントRabMAb

Anti-NDUFAB1 抗体 [EPR6725] (ab181021)

Submit a review Submit a question References (3)
Western blot - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
  • Flow Cytometry (Intracellular) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
  • Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
  • Anti-NDUFAB1 antibody [EPR6725] (ab181021)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6725] to NDUFAB1
  • Suitable for: Flow Cyt (Intra), IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-NDUFAB1 antibody [EPR6725]
    NDUFAB1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6725] to NDUFAB1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IP, IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Molt-4, A431 and HepG2 cell lysate. Human heart and kidney tissue. HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181021の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
IP
1/60.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
特記事項
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
IP
1/60.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.

ターゲット情報

  • 機能

    Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain.

  • 配列類似性

    Contains 1 acyl carrier domain.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ACP antibody
      • ACPM_HUMAN antibody
      • Acyl carrier protein antibody
      • Acyl carrier protein mitochondrial antibody
      • CI SDAP antibody
      • CI-SDAP antibody
      • FASN 2A antibody
      • FASN2A antibody
      • MGC65095 antibody
      • Mitochondrial acyl carrier protein antibody
      • mitochondrial antibody
      • NADH dehydrogenase (ubiquinone) 1 alpha/beta subcomplex 1 8kDa antibody
      • NADH dehydrogenase (ubiquinone) 1 alpha/beta subcomplex 1 antibody
      • NADH ubiquinone oxidoreductase 9.6 kDa subunit antibody
      • NADH ubiquinone oxidoreductase SDAP subunit antibody
      • NADH-ubiquinone oxidoreductase 9.6 kDa subunit antibody
      • NDUFAB 1 antibody
      • ndufab1 antibody
      • SDAP antibody
      see all

    画像

    • Western blot - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      Western blot - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      All lanes : Anti-NDUFAB1 antibody [EPR6725] (ab181021) at 1/10000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 17 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

      Immunohistochemical analysis of paraffin embedded Human heart tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      Flow Cytometry (Intracellular) - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

      Intracellular Flow Cytometry analysis of HepG2 cells labeling NDUFAB1 using ab181021 at a 1/160 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. 

       

       

    • Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] (ab181021)

      Lysate from HepG2 cells was immunoprecipitated with ab181021 at a 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

    • Anti-NDUFAB1 antibody [EPR6725] (ab181021)
      Anti-NDUFAB1 antibody [EPR6725] (ab181021)

    参考文献 (3)

    ab181021 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181021 は 3 報の論文で使用されています。

    • Fu Q  et al. Proteomics reveals MRPL4 as a high-risk factor and a potential diagnostic biomarker for prostate cancer. Proteomics 22:e2200081 (2022). PubMed: 36059095
    • Jing YY  et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978
    • Majmudar JD  et al. 4'-Phosphopantetheine and long acyl chain-dependent interactions are integral to human mitochondrial acyl carrier protein function. Medchemcomm 10:209-220 (2019). PubMed: 30881609

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