Name: Anti-NDUFA9 antibody [20C11B11B11]
SKU: ab14713
Rating: 4 (8 reviews)

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Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

Key features and details

Mouse monoclonal [20C11B11B11] to NDUFA9
Suitable for: IHC-P, WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Cow, Human
Isotype: IgG1

製品名

Anti-NDUFA9 antibody [20C11B11B11]
製品の詳細

Mouse monoclonal [20C11B11B11] to NDUFA9
由来種

Mouse
アプリケーション

適用あり: IHC-P, WB, Flow Cytmore details
種交差性

交差種: Mouse, Rat, Cow, Human
免疫原

Tissue, cells or virus corresponding to Cow NDUFA9.
ポジティブ･コントロール
- WB: WI38 and NIH 3T3 whole cell lysates, human testis tissue lysate and human, cow, rat and mouse heart mitochondria. IHC-P: Human spinal column tissue. Flow Cyt: HepG2 cells.
特記事項

This monoclonal antibody to NDUFA9 has been knockout validated in Western blot. The expected band for NDUFA9 was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
精製度

IgG fraction
特記事項（精製）

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
ポリ/モノ

モノクローナル
クローン名

20C11B11B11
アイソタイプ

IgG1
軽鎖の種類

kappa
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab14713の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P	(2)	Use at an assay dependent concentration.
WB	(5)	Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40 kDa).
Flow Cyt		Use 1-2µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40 kDa).
Flow Cyt Use 1-2µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

機能

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
配列類似性

Belongs to the complex I NDUFA9 subunit family.
細胞内局在

Mitochondrion matrix.
Target information above from: UniProt accession Q16795 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 404188 Cow
- Entrez Gene: 4704 Human
- Entrez Gene: 66108 Mouse
- Entrez Gene: 362440 Rat
- Omim: 603834 Human
- SwissProt: P34943 Cow
- SwissProt: Q16795 Human
- SwissProt: Q9DC69 Mouse
- SwissProt: Q5BK63 Rat
- Unigene: 75227 Human
- Unigene: 29939 Mouse
- Unigene: 107963 Rat
see all
別名
- CI-39kD antibody
- Complex I subunit NDUFA9 antibody
- Complex I-39kD antibody
- mitochondrial antibody
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 antibody
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial antibody
- NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9 antibody
- NADH-ubiquinone oxidoreductase 39 kDa subunit antibody
- NDUA9_HUMAN antibody
- NDUFA9 antibody
see all

Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NDUFA9 knockout HAP1 cell lysate (20 µg)
Lane 3: WI38 cell lysate (20 µg)
Lane 4: NIH3T3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab14713 observed at 40 kDa. Red - loading control, ab176560, observed at 52 kDa.

ab14713 was shown to specifically react with NDUFA9 in wild-type HAP1 cells. No band was observed when NDUFA9 knockout HAP1 samples were used. Wild-type and NDUFA9 knockout samples were subjected to SDS-PAGE. ab14713 and ab176560 (loading control to alpha tubulin) were diluted at 1μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

ab14713 a 1/100 dilution staining NDUFA9 in Human spinal column tissue by Immunohistochemistry (Formalin/PFA-Fixed paraffin-embedded sections). Antibody was incubated with the sample for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse secondary (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temperature. Sections were washed x3 in PBST and peroxidase activity was demonstrated using kit. Antigen retrieval was performed by 1 minute of pressure cooking with 1 mmol EDTA pH 8.0.
Flow Cytometry - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

Overlay histogram showing HepG2 cells stained with ab14713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14713, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

All lanes : Anti-NDUFA9 antibody [20C11B11B11] (ab14713) at 1 µg/ml

Lane 1 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 20 minutes

The band observed at 36 kDa could potentially be a cleaved form of NDUFA9 due to the presence of a 35 amino acid transit peptide.
Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

All lanes : Anti-NDUFA9 antibody [20C11B11B11] (ab14713)

Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg

Secondary
All lanes : Goat anti-Mouse IgG

Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?

Mouse on Mouse staining protocol

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab14713 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14713 は 246 報の論文で使用されています。

Hyun M et al. Melatonin protects against cadmium-induced oxidative stress via mitochondrial STAT3 signaling in human prostate stromal cells. Commun Biol 6:157 (2023). PubMed: 36750754
Van Haute L et al. TEFM variants impair mitochondrial transcription causing childhood-onset neurological disease. Nat Commun 14:1009 (2023). PubMed: 36823193
Chemello F et al. Dysfunctional mitochondria accumulate in a skeletal muscle knockout model of Smn1, the causal gene of spinal muscular atrophy. Cell Death Dis 14:162 (2023). PubMed: 36849544
Webb BD et al. Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency. Elife 12:N/A (2023). PubMed: 36881526
Qi X et al. Expanding DdCBE-mediated targeting scope to aC motif preference in rat. Mol Ther Nucleic Acids 32:1-12 (2023). PubMed: 36942261

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-9 of 9 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HEK293)

Specification

HEK293

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

Neutral buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 23 2019

Application

Western blot

Sample

Mouse Cell lysate - other (Mitochondrial extract)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Mitochondrial extract

Blocking step

Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 07 2018

Application

Western blot

Sample

Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

50 µg

Specification

AML12 hepatocytes, mitochondrial fraction

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 07 2017

Application

Western blot

Sample

American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

50 µg

Specification

fibroblasts, mitochondrial fraction

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 07 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Ventana CC1 (pH8.5)

Permeabilization

Specification

Kidney

Blocking step

Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 23 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (6-week old testes)

Specification

6-week old testes

Fixative

4% PFA

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium

Permeabilization

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Qing Wen

Verified customer

投稿 Jan 03 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK 293T)

Loading amount

20 µg

Specification

HEK 293T

Gel Running Conditions

Reduced Denaturing (10% Bis Tris)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Brian Koss

Verified customer

投稿 Dec 01 2011

Application

Western blot

Sample

Mouse Tissue lysate - other (Liver Mitochondria)

Loading amount

20 µg

Specification

Liver Mitochondria

Gel Running Conditions

Reduced Denaturing (10% Bis Trix)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 23 2011

Question

Dear Tech support

Are antibodies tested for WB expected to work with BN-PAGE method and is it guaranteed?

More specificaly I'm asking about the following antibodies:

ab14713
ab55521
ab110244
ab14715
ab14745
ab14705

As always, Thank you for your kind suppot.
Reagrds,

Abcam community

Verified customer

Asked on Aug 08 2012

Answer

Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:

ab14713
ab110244
ab14715
ab14745
ab14705

These have been tested in blue native gel. These antibodies are also frequently referenced in the literature for Blue Native. An example is a paper attached showing these in BNPAGE.

The other antibody, ab55521 has not been tested in blue native gel. We do produce many products in house, but also source many externally. Therefore all our antibodieswill have been tested in different ways. Therefore, if an antibody has been tested in western blot, this does not mean it has also been tested in blue native gel. In this case, if you have the same question regarding any of our other antibodies, please do not hesitate to contact us again and we will be happy to confirm the information for you.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to let me know.

Abcam Scientific Support

Answered on Aug 08 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

クローン名

アイソタイプ

軽鎖の種類

研究分野

関連製品

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (246)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-NDUFA9 antibody [20C11B11B11]

Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NDUFA9 antibody [20C11B11B11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NDUFA9 antibody [20C11B11B11]

Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]