Anti-NDUFA8 抗体 [EPR15303] (ab184952)
![Western blot - Anti-NDUFA8 antibody [EPR15303] (ab184952)](https://www.abcam.co.jp/ps/products/184/ab184952/Images/ab184952-217636-anti-ndufa8-antibody-epr15303-western-blot.jpg "Western blot - Anti-NDUFA8 antibody [EPR15303] (ab184952)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15303] to NDUFA8
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NDUFA8 antibody [EPR15303]
NDUFA8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15303] to NDUFA8 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal heart, Human fetal muscle, MCF7 and HepG2 lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15303 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184952の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/50 - 1/70.
|
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| WB |
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).
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| 特記事項 |
|---|
|
IP
1/50 - 1/70. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa). |
ターゲット情報
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機能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
配列類似性
Belongs to the complex I NDUFA8 subunit family.
Contains 2 CHCH domains. -
ドメイン
Contains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds. -
翻訳後修飾
May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions. -
細胞内局在
Mitochondrion. Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 4702 Human
- Omim: 603359 Human
- SwissProt: P51970 Human
- Unigene: 495039 Human
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別名
- CI 19KD antibody
- CI PGIV antibody
- CI-19kD antibody
see all
画像
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Western blot - Anti-NDUFA8 antibody [EPR15303] (ab184952)All lanes : Anti-NDUFA8 antibody [EPR15303] (ab184952) at 1/20000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal muscle lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (ab184952)](https://www.abcam.co.jp/ps/products/184/ab184952/Images/ab184952-217639-anti-ndufa8-antibody-epr15303-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (ab184952)Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal heart lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-NDUFA8 antibody [EPR15303] (ab184952)](https://www.abcam.co.jp/ps/products/184/ab184952/Images/ab184952-3-benefits-of-recombinant-antibodies.png)
Anti-NDUFA8 antibody [EPR15303] (ab184952)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab184952 は 2 報の論文で使用されています。
- Zapata-Bustos R et al. Altered Transcription Factor Expression Responses to Exercise in Insulin Resistance. Front Physiol 12:649461 (2021). PubMed: 33897458
- Habich M et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). PubMed: 30650365