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AB254732

Anti-NCOA7 抗体

Anti-NCOA7 antibody

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(1 Publication)

Rabbit Polyclonal NCOA7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCOA7 aa 550-700.

別名を表示する

ERAP140, ESNA1, Nbla00052, Nbla10993, NCOA7, Nuclear receptor coactivator 7, 140 kDa estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)

Paraffin-embedded human cerebral cortex tissue stained for NCOA7 using ab254732 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)

Paraffin-embedded human urinary bladder tissue stained for NCOA7 using ab254732 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NCOA7 antibody (AB254732)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NCOA7 antibody (AB254732)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NCOA7 using ab254732 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)

Paraffin-embedded human testis tissue stained for NCOA7 using ab254732 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB254732)

Paraffin-embedded human cerebellum tissue stained for NCOA7 using ab254732 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-NCOA7 antibody (AB254732)
  • WB

Supplier Data

Western blot - Anti-NCOA7 antibody (AB254732)

All lanes:

Western blot - Anti-NCOA7 antibody (ab254732) at 0.4 µg/mL

All lanes:

HDLM-2 cell lysate

Predicted band size: 107 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human NCOA7 aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q8NI08

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nuclear receptor coactivator 7 (NCOA7) also known as ERAP plays a role in transcriptional regulation by interacting with nuclear hormone receptors to enhance their transcriptional activity. It has a molecular mass of approximately 100 kDa. NCOA7 is expressed in various tissues with significant levels in the brain and endocrine organs. The protein influences the transcriptional activity of hormone receptors by modulating chromatin structure and accessibility.
Biological function summary

NCOA7 influences cellular processes through interactions with nuclear receptors. It forms a complex with these receptors to modulate gene expression. This protein engages in the regulation of genes that are involved in cell development and homeostasis. NCOA7's activity can influence metabolic and signaling processes that are critical for maintaining proper cellular environment and function.

Pathways

NCOA7 associates with important biological pathways including nuclear hormone receptor signaling and lipid metabolism. It interacts with proteins like peroxisome proliferator-activated receptors (PPARs) and estrogen receptors (ERs) in these pathways. These interactions facilitate the regulation of genes involved in lipid storage energy balance and other metabolic functions.

NCOA7 has implications in conditions such as breast cancer and neurodegenerative diseases. In breast cancer its interaction with estrogen receptors can influence tumor growth and development. NCOA7 is also connected to conditions like Alzheimer's disease through its influence on brain tissues potentially interacting with proteins like amyloid-beta precursor protein which is relevant to the disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
See full target information NCOA7
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:4535-4547 PubMed37750019

2023

Tetraspanin 1 regulates papillary thyroid tumor growth and metastasis through c-Myc-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihua Han,Changming Xie,Bo Liu,Yan Wang,Rui Pang,Wen Bi,Rinan Sheng,Guoqing He,Lingyu Kong,Jiawei Yu,Zhaoming Ding,Lili Chen,Jinliang Jia,Jiewu Zhang,Chunlei Nie
View all publications
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代替製品

Primary Antibodies

AB224481

Anti-NCOA7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA7 antibody (AB224481)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Cell Lines & Lysates

AB267145

Human NCOA7 knockout A549 cell line

Sanger Sequencing - Human NCOA7 knockout A549 cell line (AB267145)

  • 0
  • 0
Proteins & Peptides

AB285976

Recombinant EN-TEV Protease protein

Bioactive

Size Exclusion Chromatography - Recombinant EN-TEV Protease protein (AB285976)

  • 0
  • 0
Cell Lines & Lysates

AB263770

Human SQSTM1 (p62) knockout HEK-293T cell lysate

Western blot - Human SQSTM1 (p62) knockout HEK-293T cell lysate (AB263770)

  • 0
  • 0

Abcam product promise

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