AB151553

Anti-NCOA62/SNW1 抗体 [EPR9988(B)]

Anti-NCOA62/SNW1 antibody [EPR9988(B)]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NCOA62/SNW1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

別名を表示する

SKIIP, SKIP, SNW1, SNW domain-containing protein 1, Nuclear protein SkiP, Nuclear receptor coactivator NCoA-62, Ski-interacting protein

1 Images

Unknown

Western blot - Anti-NCOA62/SNW1 antibody [EPR9988(B)] (AB151553)

All lanes:

Western blot - Anti-NCOA62/SNW1 antibody [EPR9988(B)] (ab151553) at 1/1000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9988(B)

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein NCOA62 also known as SNW1 or Ski-interacting protein functions as a transcriptional coactivator in the cell nucleus. This protein with a mass of approximately 60 kDa is involved in various transcriptional activities. You will find it expressed in many tissue types but especially in the highly proliferative ones like the liver brain and reproductive tissues. The protein associates with other nuclear receptors and transcription factors to modulate gene expression.

Biological function summary

NCOA62/SNW1 interacts with several transcription complexes playing an important role in modulating transcriptional signals. It acts as a scaffolding molecule facilitating the assembly and function of specific transcriptional complexes. It binds with various partners including the Smad and p53 proteins partaking in cell cycle regulation and DNA repair processes. Its role in transcription complex suggests its involvement in essential cellular functions.

Pathways

NCOA62/SNW1 plays a role in the TGF-beta signaling pathway and the p53 pathway. These pathways are critical for regulating cell growth differentiation and apoptosis. Within the TGF-beta pathway the protein aids in transmitting signals from the cell membrane to the nucleus by interacting with Smad proteins. It also influences the p53 pathway by modulating p53 activity an important protein recognized for its role in maintaining genomic integrity and responding to cellular stress.

NCOA62/SNW1 displays significant involvement in several medical conditions including cancer and neurodegenerative diseases. In cancer it influences the cell cycle and apoptosis by interacting with Smad and p53 potentially leading to uncontrolled cell growth when dysregulated. Regarding neurodegenerative disorders alterations in NCOA62/SNW1 expression or function could contribute to neuronal cell death by disrupting normal cell signaling mechanisms connected possibly through interaction with p53 well-known for its involvement in cell death and survival pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Required for the specific splicing of CDKN1A pre-mRNA; the function probably involves the recruitment of U2AF2 to the mRNA. May recruit PPIL1 to the spliceosome. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in transcriptional regulation. Modulates TGF-beta-mediated transcription via association with SMAD proteins, MYOD1-mediated transcription via association with PABPN1, RB1-mediated transcriptional repression, and retinoid-X receptor (RXR)- and vitamin D receptor (VDR)-dependent gene transcription in a cell line-specific manner probably involving coactivators NCOA1 and GRIP1. Is involved in NOTCH1-mediated transcriptional activation. Binds to multimerized forms of Notch intracellular domain (NICD) and is proposed to recruit transcriptional coactivators such as MAML1 to form an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ to form a transcriptional activation complex by releasing SNW1 and redundant NOTCH1 NICD.. (Microbial infection) Is recruited by HIV-1 Tat to Tat : P-TEFb : TAR RNA complexes and is involved in Tat transcription by recruitment of MYC, MEN1 and TRRAP to the HIV promoter.. (Microbial infection) Proposed to be involved in transcriptional activation by EBV EBNA2 of CBF-1/RBPJ-repressed promoters.

See full target information SNW1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 41:101893 PubMed39760097

2025

Construction and evaluation of a prognostic model of autophagy-related genes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yutao He,Bin Du,Weiran Liao,Wei Wang,Jifeng Su,Chen Guo,Kai Zhang,Zhitian Shi

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCOA62/SNW1 抗体 [EPR9988(B)]

Anti-NCOA62/SNW1 antibody [EPR9988(B)]

Western blot - Anti-NCOA62/SNW1 antibody [EPR9988(B)] (AB151553)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise