Anti-NCOA4 抗体 (ab86707)
Key features and details
- Rabbit polyclonal to NCOA4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NCOA4 antibody
NCOA4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NCOA4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 564 and 614 of Human NCOA4 (NP_005428.1).
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ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86707の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
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| IP |
Use at 10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa. |
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IP
Use at 10 µg/mg of lysate. |
ターゲット情報
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機能
Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. -
組織特異性
Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis. -
関連疾患
Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas. - Information by UniProt
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参照データベース
- Entrez Gene: 8031 Human
- Entrez Gene: 27057 Mouse
- Omim: 601984 Human
- SwissProt: Q13772 Human
- Unigene: 643658 Human
- Unigene: 709644 Human
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別名
- 70 kDa androgen receptor activator antibody
- 70 kDa androgen receptor coactivator antibody
- 70 kDa AR activator antibody
see all
画像
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Western blot - Anti-NCOA4 antibody (ab86707)All lanes : Anti-NCOA4 antibody (ab86707) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 70 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 171 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with an exposure time of 3 minutes. -
Immunoprecipitation - Anti-NCOA4 antibody (ab86707)Western Blot detection of NCOA4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86707 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab86707 は 25 報の論文で使用されています。
- Liu Y et al. Stimulation of Hepatic Ferritinophagy Mitigates Irp2 Depletion-Induced Anemia. Antioxidants (Basel) 12:N/A (2023). PubMed: 36978814
- Shi H et al. Susceptibility of cervical cancer to dihydroartemisinin-induced ferritinophagy-dependent ferroptosis. Front Mol Biosci 10:1156062 (2023). PubMed: 37065442
- Wu X et al. Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway. Cell Death Dis 14:577 (2023). PubMed: 37648705
- Liu J et al. NCOA4-Mediated Ferroptosis in Bronchial Epithelial Cells Promotes Macrophage M2 Polarization in COPD Emphysema. Int J Chron Obstruct Pulmon Dis 17:667-681 (2022). PubMed: 35386390
- Ni Z et al. Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity. Cell Death Dis 13:579 (2022). PubMed: 35787614