Anti-NCOA4 抗体 [439CT10.1.2] (ab62495)
![Western blot - Anti-NCOA4 antibody [439CT10.1.2] (ab62495)](https://www.abcam.co.jp/ps/products/62/ab62495/Images/ab62495-75831-anti-ncoa4-antibody-439ct1012-western-blot.jpg "Western blot - Anti-NCOA4 antibody [439CT10.1.2] (ab62495)")
Key features and details
- Mouse monoclonal [439CT10.1.2] to NCOA4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-NCOA4 antibody [439CT10.1.2]
NCOA4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [439CT10.1.2] to NCOA4 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human NCOA4 aa 400 to the C-terminus (C terminal).
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ポジティブ･コントロール
- This antibody gave a positive signal in HEK293 and PC3 whole cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Concentrated Culture Supernatant -
ポリ/モノ
モノクローナル -
クローン名
439CT10.1.2 -
ミエローマ
Sp2/0 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62495の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Use 3% milk as blocking agent.
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| 特記事項 |
|---|
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WB
1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Use 3% milk as blocking agent. |
ターゲット情報
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機能
Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. -
組織特異性
Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis. -
関連疾患
Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas. - Information by UniProt
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参照データベース
- Entrez Gene: 8031 Human
- Omim: 601984 Human
- SwissProt: Q13772 Human
- Unigene: 643658 Human
- Unigene: 709644 Human
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別名
- 70 kDa androgen receptor activator antibody
- 70 kDa androgen receptor coactivator antibody
- 70 kDa AR activator antibody
see all
画像
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Western blot - Anti-NCOA4 antibody [439CT10.1.2] (ab62495)All lanes : Anti-NCOA4 antibody [439CT10.1.2] (ab62495) at 1/100 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using 3% milk as the blocking agent.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab62495 は 2 報の論文で使用されています。
- Wu X et al. Aqueous-soluble components of sporoderm-removed Ganoderma lucidum spore powder promote ferroptosis in oral squamous cell carcinoma. Chin J Cancer Res 35:176-190 (2023). PubMed: 37180834
- Luo H & Zhang R Icariin enhances cell survival in lipopolysaccharide-induced synoviocytes by suppressing ferroptosis via the Xc-/GPX4 axis. Exp Ther Med 21:72 (2021). PubMed: 33365072