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Anti-NCF1/p47-phox (phospho S304) 抗体 (ab63554)

Submit a review Q&A (3)References (4)
Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)

    Key features and details

    • Rabbit polyclonal to NCF1/p47-phox (phospho S304)
    • Suitable for: WB
    • Reacts with: Human, African green monkey
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-NCF1/p47-phox (phospho S304) antibody
      NCF1/p47-phox 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to NCF1/p47-phox (phospho S304)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human, African green monkey

    • 免疫原

      Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S304).
      Sequence:

      R-S-Sp-I-R

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • COS7 cells

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab63554の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
    特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    ターゲット情報

    • 機能

      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

    • 関連疾患

      Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

    • 配列類似性

      Contains 1 PX (phox homology) domain.
      Contains 2 SH3 domains.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 47 kDa autosomal chronic granulomatous disease protein antibody
        • 47 kDa neutrophil oxidase factor antibody
        • NADPH oxidase organizer 2 antibody
        • NCF 47K antibody
        • NCF-1 antibody
        • NCF-47K antibody
        • Ncf1 antibody
        • NCF1_HUMAN antibody
        • Neutrophil cytosol factor 1 antibody
        • Neutrophil cytosolic factor 1 antibody
        • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
        • Neutrophil NADPH oxidase factor 1 antibody
        • Nox organizer 2 antibody
        • Nox organizing protein 2 antibody
        • Nox-organizing protein 2 antibody
        • NOXO2 antibody
        • p47 phox antibody
        • p47-phox antibody
        • SH3 and PX domain containing protein 1A antibody
        • SH3 and PX domain-containing protein 1A antibody
        • SH3PXD1A antibody
        see all

      画像

      • Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
        Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
        All lanes : Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution

        Lane 1 : extracts from COS7 cells treated
        with UV (15mins)
        Lane 2 : A549 cells extracts
        Lane 3 : A549 cells extracts with immunizing peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      参考文献 (4)

      ab63554 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab63554 は 4 報の論文で使用されています。

      • Song P  et al. Geniposide protects against sepsis-induced myocardial dysfunction through AMPKa-dependent pathway. Free Radic Biol Med 152:186-196 (2020). PubMed: 32081748
      • Guo Y  et al. D-4F Ameliorates Contrast Media-Induced Oxidative Injuries in Endothelial Cells via the AMPK/PKC Pathway. Front Pharmacol 11:556074 (2020). PubMed: 33658920
      • Sowrirajan B  et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). PubMed: 28240310
      • Leverence JT  et al. Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: Role of PKC?-mediated p47phox phosphorylation. Chem Biol Interact : (2010). WB ; Human . PubMed: 20920494

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      Would like to use antibody in Flow Cytometry.

      Read More
      Answer

      DISCOUNT CODE: XXXXXXX
      Expiration date: 31-05-2013
      Value: £235

      I am very pleased to hear you would like to accept our offer and test ab63554 in Flow Cytometry. This code will give you ab63554 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

      Read More

      Question

      Are there any antibodies available that detect the phosphorylated form of p47phox (a regulatory NADPH oxidase subunit) in mouse tissue? I have attached a recent paper which has published in antioxidants and redox signaling. Authors used an antibody from your company for the detection of phospho p47phox in cells extracted from humans.



      Thanks,

      Read More
      Answer

      Thank you for contacting us.

      Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

      The antibodies we have are; ab74095, ab63331, ab111855, ab115564, ab795, ab63554, ab107125. Please check the datasheets and let me know, if you want me to create a performa invoice for you.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      No signal detected in blots of rat cell lysates; ab74095 gives expected signal with same samples

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***** for ab74095. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

      Read More

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