Anti-NCF1/p47-phox 抗体 (ab795)
Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-NCF1/p47-phox antibody
NCF1/p47-phox 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NCF1/p47-phox -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Pig
交差が予測される動物種: Rat, Rabbit, Cow -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: U937, Daudi, and U251 cell lysates, Mouse thymus and pig spleen tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab795の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
関連疾患
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
配列類似性
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 653361 Human
- Entrez Gene: 17969 Mouse
- Entrez Gene: 114553 Rat
- Omim: 608512 Human
- SwissProt: P14598 Human
- SwissProt: Q09014 Mouse
- SwissProt: F1M707 Rat
- Unigene: 647047 Human
see all -
別名
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
画像
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Lane 1 : Anti-NCF1/p47-phox antibody (ab795) at 0.2 µg/ml
Lane 2 : Anti-NCF1/p47-phox antibody (ab795) at 0.01 µg/ml
Lanes 3-4 : Anti-NCF1/p47-phox antibody (ab795) at 0.3 µg/ml
Lane 1 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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All lanes : Anti-NCF1/p47-phox antibody (ab795) at 1 µg/ml
Lane 1 : Mouse thymus lysate
Lane 2 : Pig spleen lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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Anti-NCF1/p47-phox antibody (ab795) at 1.5 µg/ml +
Recombinant Human NCF1/p47-phox protein (ab98054) at 0.1 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab795 は 20 報の論文で使用されています。
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
- Lavier J et al. High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice. Acta Physiol (Oxf) 233:e13700 (2021). PubMed: 34089562
- Wang QS et al. NaHS or Lovastatin Attenuates Cyclosporine A-Induced Hypertension in Rats by Inhibiting Epithelial Sodium Channels. Front Pharmacol 12:665111 (2021). PubMed: 34122084
- Luo LP et al. Shenkang Injection and Its Three Anthraquinones Ameliorates Renal Fibrosis by Simultaneous Targeting IƙB/NF-ƙB and Keap1/Nrf2 Signaling Pathways. Front Pharmacol 12:800522 (2021). PubMed: 35002735
- Zhuang J et al. Age-related accumulation of advanced oxidation protein products promotes osteoclastogenesis through disruption of redox homeostasis. Cell Death Dis 12:1160 (2021). PubMed: 34907153