Anti-NCF1/p47-phox 抗体 (ab166930)
Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NCF1/p47-phox antibody
NCF1/p47-phox 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NCF1/p47-phox -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Guinea pig, Cow, Dog, Pig, Chinese hamster
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免疫原
Synthetic peptide corresponding to Mouse NCF1/p47-phox aa 217-230 (internal sequence) (Cysteine residue).
Sequence:DEAEDPDPNYAGEP
Database link: NP_035006.3 -
ポジティブ･コントロール
- WB: Daudi cell lydates ICC/IF: RAW264.7 and NIH3T3 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab166930 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB | (2) |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 45 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 45 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
関連疾患
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
配列類似性
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 653361 Human
- Entrez Gene: 17969 Mouse
- Entrez Gene: 114553 Rat
- Omim: 608512 Human
- SwissProt: P14598 Human
- SwissProt: Q09014 Mouse
- SwissProt: F1M707 Rat
- Unigene: 647047 Human
see all -
別名
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
画像
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Western blot - Anti-NCF1/p47-phox antibody (ab166930)Anti-NCF1/p47-phox antibody (ab166930) at 1 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 45 kDaDetected by chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (ab166930)Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL). -
Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (ab166930)Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab166930 は 6 報の論文で使用されています。
- Zhang W et al. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 13:22588-22610 (2021). PubMed: 34584017
- Xirouchaki CE et al. Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance. Sci Adv 7:eabl4988 (2021). PubMed: 34910515
- Zhou Y et al. Lipoxin A4 attenuates uric acid-activated, NADPH oxidase-dependent oxidative stress by interfering with translocation of p47phox in human umbilical vein endothelial cells. Exp Ther Med 20:1682-1692 (2020). PubMed: 32765680
- Lin YS et al. HIF-1a-Mediated, NADPH Oxidase-Derived ROS Contributes to Laryngeal Airway Hyperreactivity Induced by Intermittent Hypoxia in Rats. Front Physiol 11:575260 (2020). PubMed: 33117193
- Zeng X et al. A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice. J Immunol Res 2020:6687143 (2020). PubMed: 33426093
- Bernardini A et al. Carotid body type I cells engage flavoprotein and Pin1 for oxygen sensing. Am J Physiol Cell Physiol 318:C719-C731 (2020). PubMed: 31967857