AB181090

Anti-NCF1/p47-phox 抗体 [EPR13134-23]

Anti-NCF1/p47-phox antibody [EPR13134-23]

RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal NCF1/p47-phox antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 11 publications.

別名を表示する

NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling NCF1/p47-phox with ab181090 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

Western blot analysis of Raji cell lysate immunoprecipitated with ab181090 at 1/20 dilution (Lane 1). Lane 2 : Negative control.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

All lanes:

Western blot - Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090) at 1/2000 dilution

Lane 1:

Raji cell lysate at 20 µg

Lane 2:

Ramos cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 45 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13134-23

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.

Biological function summary

The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.

Pathways

The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.

Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 2547247, PubMed : 2550933, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed : 12732142, PubMed : 19801500).

See full target information NCF1

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0331657 PubMed40920799

2025

Interferon-gamma 1b-induced gene expression alters neutrophil function in patients with chronic granulomatous disease.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel R Ambruso,Natalie J Briones,Alexander D Tran,Bridget Sanford,Christine Childs,Ben Z Katz,Michael Ellison,Richard B Johnston,Kenneth L Jones

Frontiers in molecular neuroscience 17:1459358 PubMed39664113

2024

Effect of histone demethylase KDM5B on long-term cognitive impairment in neonatal rats induced by sevoflurane.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Wang,Yun Chen,Mengxiao Zhang,Chengdong Yuan,Yu Zhang,Xingjian Liu,Yi Zhang,Xiaoli Liang

Redox biology 75:103254 PubMed38968922

2024

NADPH oxidase 2 activity disrupts Calmodulin/CaMKIIα complex via redox modifications of CaMKIIα-contained Cys30 and Cys289: Implications in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Filippo Pullara,Madison C Forsmann,Ignacio J General,Joseph C Ayoob,Emily Furbee,Sandra L Castro,Xiaoping Hu,J Timothy Greenamyre,Roberto Di Maio

Journal of translational medicine 22:497 PubMed38796413

2024

Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

MingXian Chen,Yu Chen,Rui Fu,SaiYue Liu,HaiXia Li,TangBiao Shen

Drug development research 84:1008-1017 PubMed37114746

2023

All-trans retinoic acid inhibits oxidative stress via ACE2/Ang (1-7)/MasR pathway in renal tubular epithelial cells stimulated with high glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Zhu,Zhixiang Mou,Wei Han,Lan Chen

Journal of viral hepatitis 29:627-636 PubMed35633088

2022

TRPM2 regulates autophagy to participate in hepatitis B virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Chen,Liying Zhu,Xingyu Lu,Xiaoyu Ming,Baoshan Yang

Neurobiology of disease 170:105754 PubMed35577065

2022

NADPH oxidase 2 activity in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew T Keeney,Eric K Hoffman,Kyle Farmer,Christopher R Bodle,Marco Fazzari,Alevtina Zharikov,Sandra L Castro,Xiaoping Hu,Amanda Mortimer,Julia K Kofler,Eugenia Cifuentes-Pagano,Patrick J Pagano,Edward A Burton,Teresa G Hastings,J Timothy Greenamyre,Roberto Di Maio

Cell death discovery 8:22 PubMed35022384

2022

Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats.

Applications

Unspecified application

Species

Unspecified reactive species

Juexiao Gong,Man Luo,Yonghong Yong,Shan Zhong,Peng Li

Experimental and therapeutic medicine 23:120 PubMed34970343

2022

Bazedoxifene activates the angiotensin II-induced HUVEC hypertension model by targeting SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Yu,Jin Zhao,Baotang Liu

International journal of molecular sciences 22: PubMed33572690

2021

Influence of Antiplatelet Agents on the Lipid Composition of Platelet Plasma Membrane: A Lipidomics Approach with Ticagrelor and Its Active Metabolite.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Lagoutte-Renosi,Florentin Allemand,Christophe Ramseyer,Vahideh Rabani,Siamak Davani

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCF1/p47-phox 抗体 [EPR13134-23]

Anti-NCF1/p47-phox antibody [EPR13134-23]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

Western blot - Anti-NCF1/p47-phox antibody [EPR13134-23] (AB181090)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (11)

Abcam product promise