Anti-NCAPG2 抗体 (ab70350)
Key features and details
- Rabbit polyclonal to NCAPG2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NCAPG2 antibody
NCAPG2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NCAPG2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide corresponding to a region between C terminal residues 1100 -1143 of human NCAPG2. (AAH43404.1)
-
ポジティブ･コントロール
- WB: HeLa whole cell lysate (ab150035) IP: HeLa whole cell extract
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70350の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
|
| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa). |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
-
関連性
NCAPG2 is 1 of 3 non-SMC subunits that define condensin II. Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II. -
細胞内局在
nucleus -
参照データベース
- Entrez Gene: 54892 Human
- Omim: 608532 Human
- SwissProt: Q86XI2 Human
-
別名
- CAP G2 antibody
- Chromosome associated protein G2 antibody
- Condensin 2 complex subunit G2 antibody
see all
画像
-
Immunoprecipitation - Anti-NCAPG2 antibody (ab70350)ab70350 immunoprecipitating NCAPG2 at 6 µg per reaction. Lane 1: ab70350 in HeLa whole cell extract (0.5 or 1 mg per IP reaction; 20% of IP loaded). Lane 2: IgG control. For blotting immunoprecipitated NCAPG2, ab70350 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
-
Western blot - Anti-NCAPG2 antibody (ab70350)All lanes : Anti-NCAPG2 antibody (ab70350) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 131 kDa
Observed band size: 131 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab70350 は 9 報の論文で使用されています。
- Wang M et al. miR-638 suppresses cervical cancer progression by inhibiting NCAPG2 under the treatment of Tetrandrine. Histol Histopathol 39:497-509 (2024). PubMed: 37702425
- Dai HT et al. microRNA-375 inhibits the malignant behaviors of hepatic carcinoma cells by targeting NCAPG2. Neoplasma 69:16-27 (2022). PubMed: 34818025
- Jiang S et al. NCAPG2 Maintains Cancer Stemness and Promotes Erlotinib Resistance in Lung Adenocarcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 36139554
- Ward JR et al. Condensin I and condensin II proteins form a LINE-1 dependent super condensin complex and cooperate to repress LINE-1. Nucleic Acids Res 50:10680-10694 (2022). PubMed: 36169232
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Deutschman E et al. Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses. J Cell Sci 132:N/A (2019). PubMed: 31653782
- Abramo K et al. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol 21:1393-1402 (2019). PubMed: 31685986
- Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . PubMed: 20622854
- Centore RC et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . PubMed: 20932472