Anti-NCAM1 抗体 [RNL-1] (ab9018)
Key features and details
- Mouse monoclonal [RNL-1] to NCAM1
- Suitable for: ICC, IHC-FoFr
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-NCAM1 antibody [RNL-1]
NCAM1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RNL-1] to NCAM1 -
由来種
Mouse -
特異性
Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180. -
アプリケーション
適用あり: ICC, IHC-FoFrmore details
適用なし: Flow Cyt -
種交差性
交差種: Rat, Human -
免疫原
Tissue, cells or virus corresponding to Human NCAM1. This antibody was raised against the small cell lung cancer cell line NCI-H82.
Database link: P13591 -
ポジティブ・コントロール
- IHC-P: Mouse brain tissue. IHC-Fr: Rat brain tissue. ICC/IF: Adult-derived human liver stem cells and hepatic stellate cells.
-
特記事項
Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
RNL-1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9018の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC | (1) |
1/100 - 1/200.
|
IHC-FoFr |
1/100 - 1/200.
|
特記事項 |
---|
ICC
1/100 - 1/200. |
IHC-FoFr
1/100 - 1/200. |
ターゲット情報
-
機能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
配列類似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 4684 Human
- Entrez Gene: 24586 Rat
- Omim: 116930 Human
- SwissProt: P13591 Human
- SwissProt: P13596 Rat
- Unigene: 503878 Human
- Unigene: 11283 Rat
-
別名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
画像
-
Immunohistochemistry of H82 cells, showing staining for NCAM1 using ab9018 (1/500).
-
Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)Image cropped from Berardis et al., Public Library of Science One, 1, e86137, Fig. 3a.; DOI: 10.1371/journal.pone.0086137 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin.
-
ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30
µ m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.NB: We recommend indirect amplification of immunofluorescence for ab9018
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (29)
ab9018 は 29 報の論文で使用されています。
- Sun JH et al. Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interaction. Neural Regen Res 17:1023-1033 (2022). PubMed: 34558529
- Lee HR et al. CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression. Arch Rheumatol 37:335-343 (2022). PubMed: 36589618
- Sanmarco LM et al. Gut-licensed IFNγ+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes. Nature 590:473-479 (2021). PubMed: 33408417
- Wang J et al. Low immune index correlates with favorable prognosis but with reduced benefit from chemotherapy in gallbladder cancer. Cancer Sci 111:219-228 (2020). PubMed: 31729088
- Rao F et al. Aligned chitosan nanofiber hydrogel grafted with peptides mimicking bioactive brain-derived neurotrophic factor and vascular endothelial growth factor repair long-distance sciatic nerve defects in rats. Theranostics 10:1590-1603 (2020). PubMed: 32042324