Anti-NCAM1 抗体 [MEM-188] (ab8233)
Key features and details
- Mouse monoclonal [MEM-188] to NCAM1
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-NCAM1 antibody [MEM-188]
NCAM1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-188] to NCAM1 -
由来種
Mouse -
特異性
CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Non human primates -
免疫原
Tissue, cells or virus corresponding to Human NCAM1. KG-1 acute myelogenous leukaemia cell line
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ポジティブ・コントロール
- Flow Cyt: Human peripheral blood.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MEM-188 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8233の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use a concentration of 0.5 - 3 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use a concentration of 0.5 - 3 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
配列類似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4684 Human
- Omim: 116930 Human
- SwissProt: P13591 Human
- Unigene: 503878 Human
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別名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
画像
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Flow cytometry analysis (surface staining) of human peripheral blood lymphocytes with ab8233 (GAM-APC).
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Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab8233 は 1 報の論文で使用されています。
- Gupta J et al. Pharmacological inhibition of p38 MAPK reduces tumor growth in patient-derived xenografts from colon tumors. Oncotarget 6:8539-51 (2015). IHC . PubMed: 25890501