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AB214436

Anti-NCAM1 抗体 [EPR2566] - BSA and Azide free

Anti-NCAM1 antibody [EPR2566] - BSA and Azide free

4

(3 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal NCAM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] - BSA and Azide free (AB214436)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] - BSA and Azide free (AB214436)

This IHC data was generated using the same anti-NCAM antibody clone, EPR2566, in a different buffer formulation (cat# ab133345).

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] - BSA and Azide free (AB214436)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] - BSA and Azide free (AB214436)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human cerebellum labelling NCAM1 with ab214436 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab214436 anti NCAM1 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2566

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB154566

Anti-NCAM1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody (AB154566)

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Primary Antibodies

AB218467

Anti-NCAM antibody [NCAM1/1496]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [NCAM1/1496] (AB218467)

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Primary Antibodies

AB218925

Anti-NCAM antibody [NCAM1/1496] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [NCAM1/1496] - BSA and Azide free (AB218925)

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Primary Antibodies

AB9018

Anti-NCAM1 antibody [RNL-1]

Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (AB9018)

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Primary Antibodies

AB216019

Anti-NCAM antibody [NCAM1/784]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [NCAM1/784] (AB216019)

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Primary Antibodies

AB200698

Anti-NCAM1 antibody [123C3.D5 + 123A8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [123C3.D5 + 123A8] (AB200698)

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Primary Antibodies

AB205500

APC Anti-NCAM1 antibody [LT56]

Flow Cytometry - APC Anti-NCAM1 antibody [LT56] (AB205500)

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Primary Antibodies

AB234222

PerCP Anti-NCAM1 antibody [LT56]

Flow Cytometry - PerCP Anti-NCAM1 antibody [LT56] (AB234222)

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Primary Antibodies

AB28335

APC Anti-NCAM1 antibody [MEM-188]

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

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Primary Antibodies

AB69763

FITC Anti-NCAM1 antibody [MEM-188]

Flow Cytometry - FITC Anti-NCAM1 antibody [MEM-188] (AB69763)

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Primary Antibodies

AB234274

PE/Cy7® Anti-NCAM1 antibody [MEM-188]

Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [MEM-188] (AB234274)

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Primary Antibodies

AB234250

FITC Anti-NCAM1 antibody [LT56]

Flow Cytometry - FITC Anti-NCAM1 antibody [LT56] (AB234250)

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Primary Antibodies

AB6123

Anti-NCAM1 antibody [ERIC-1]

Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (AB6123)

  • 0
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Primary Antibodies

AB206632

PerCP/Cy5.5® Anti-NCAM1 antibody [LT56]

Flow Cytometry - PerCP/Cy5.5® Anti-NCAM1 antibody [LT56] (AB206632)

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Primary Antibodies

AB233575

PE/Cy7® Anti-NCAM1 antibody [LT56]

Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [LT56] (AB233575)

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組合せ製品

Assay Kits

AB315307

Human NCAM1 ELISA Kit

SimpleStep

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

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Sample Prep & Detection Kits

AB269904

FluoProbes 647H Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - FluoProbes 647H Conjugation Kit (Fast) - Lightning-Link® (AB269904)

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Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

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Primary Antibodies

AB215977

Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Mass Cytometry - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (AB215977)

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Primary Antibodies

AB183322

Anti-CD14 antibody [SP192]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (AB183322)

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Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab214436 is the carrier-free version of ab133345.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Immunity 54:1594-1610.e11 PubMed34174183

2021

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Schwabenland,Henrike Salié,Jovan Tanevski,Saskia Killmer,Marilyn Salvat Lago,Alexandra Emilia Schlaak,Lena Mayer,Jakob Matschke,Klaus Püschel,Antonia Fitzek,Benjamin Ondruschka,Henrik E Mei,Tobias Boettler,Christoph Neumann-Haefelin,Maike Hofmann,Angele Breithaupt,Nafiye Genc,Christine Stadelmann,Julio Saez-Rodriguez,Peter Bronsert,Klaus-Peter Knobeloch,Thomas Blank,Robert Thimme,Markus Glatzel,Marco Prinz,Bertram Bengsch

Acta orthopaedica 84:565-70 PubMed24171689

2013

Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears.

Applications

IHC-P

Species

Human

Kirsten Lundgreen,Oystein Bjerkestrand Lian,Lars Engebretsen,Alex Scott
View all publications
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