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Anti-NCAM1 抗体 [123C3] (ab9272)

Reviews (4)Q&A (5)References (25)
Immunohistochemistry - Free Floating - Anti-NCAM1 antibody [123C3] (ab9272)
  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (ab9272)
  • Flow Cytometry - Anti-NCAM1 antibody [123C3] (ab9272)

Key features and details

  • Mouse monoclonal [123C3] to NCAM1
  • Suitable for: IHC-FrFl, IHC-Fr, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human, Zebrafish
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-NCAM1 antibody [123C3]
    NCAM1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [123C3] to NCAM1

  • 由来種

    Mouse

  • 特異性

    This antibody stains neural cell adhesion molecule (NCAM) which is present in small cell lung cancer and lung carcinoids. It also reacts with a number of non-small cell lung carcinomas and neuroendocrine and neuronal derived tissues. In addition, it is internalized after binding to its antigen on SCLC cell lines, making it an excellent reagent for tumor imaging in xenograft models. ab9272 reacts with all three isoforms of NCAM.

  • アプリケーション

    適用あり: IHC-FrFl, IHC-Fr, Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Rat, Human, Zebrafish

  • 免疫原

    Tissue, cells or virus corresponding to Human NCAM1. This antibody was raised against a small cell lung carcinoma specimen.

  • エピトープ

    This antibody recognizes an epitope in the NCAM exons 11-13 which is dependent on an intact conformation of the first fibronectin type-III homologous domain encoded by these exons.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9272の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FrFl
Use at an assay dependent concentration.
IHC-Fr (1)
1/500 - 1/1000.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/1000.
特記事項
IHC-FrFl
Use at an assay dependent concentration.
IHC-Fr
1/500 - 1/1000.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/1000.

ターゲット情報

  • 機能

    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

  • 配列類似性

    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
      • antigen recognized by monoclonal antibody 5.1H11 antibody
      • CD56 antibody
      • cell adhesion molecule, neural, 1 antibody
      • MSK 39 antibody
      • MSK39 antibody
      • N-CAM-1 antibody
      • NCAM 1 antibody
      • NCAM antibody
      • NCAM C antibody
      • NCAM-1 antibody
      • NCAM1 antibody
      • NCAM1_HUMAN antibody
      • NCAMC antibody
      • Neural cell adhesion molecule 1 antibody
      • Neural cell adhesion molecule NCAM antibody
      • OTTHUMP00000235666 antibody
      see all

    画像

    • Immunohistochemistry - Free Floating - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunohistochemistry - Free Floating - Anti-NCAM1 antibody [123C3] (ab9272)

      ab9272 at a dilution of 1/1000, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing hippocampal neuron body and processes: [A] 20x objective and [B] punctate cytoplasmic staining; 40x objective. No labeling observed following omission of primary antibody.

      Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

    • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunofluorescence staining images of 9 day old zebrafish embryos.
      ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
    • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunofluorescence staining images of 9 day old zebrafish embryos.
      ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
    • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunofluorescence staining images of 9 day old zebrafish embryos.
      ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
    • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (ab9272)
      Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (ab9272)

      Immunostaining of the neural cell adhesion molecule (NCAM) in small cell human lung cancer cell line NCI-H82.

    • Flow Cytometry - Anti-NCAM1 antibody [123C3] (ab9272)
      Flow Cytometry - Anti-NCAM1 antibody [123C3] (ab9272)
      Overlay histogram showing SH-SY5Y cells stained with ab9272 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献 (25)

    ab9272 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9272 は 25 報の論文で使用されています。

    • Islamov R  et al. New Therapy for Spinal Cord Injury: Autologous Genetically-Enriched Leucoconcentrate Integrated with Epidural Electrical Stimulation. Cells 11:N/A (2022). PubMed: 35011706
    • Lan X  et al. Bone marrow mesenchymal stem cells-derived exosomes mediate nuclear receptor coactivator-3 expression in osteoblasts by delivering miR-532-5p to influence osteonecrosis of the femoral head development. Cell Biol Int 46:2185-2197 (2022). PubMed: 36116109
    • Guo T  et al. Dual inhibition of FGFR4 and BCL-xL inhibits multi-resistant ovarian cancer with BCL2L1 gain. Aging (Albany NY) 13:19750-19759 (2021). PubMed: 34351305
    • Tong W  et al. Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33576467
    • Yoshimine S  et al. Autologous Multilayered Fibroblast Sheets Can Reinforce Bronchial Stump in a Rat Model. Semin Thorac Cardiovasc Surg N/A:N/A (2021). PubMed: 33711463
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-NCAM antibody [123C3]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 03 2012

    Western blot abreview for Anti-NCAM antibody [123C3]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (fibroblast)
    Loading amount
    100 µg
    Specification
    fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 15 2012

    Western blot abreview for Anti-NCAM antibody [123C3]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Cell lysate - whole cell (brain and whole fish lysate)
    Loading amount
    100 µg
    Specification
    brain and whole fish lysate
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 14 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-NCAM antibody [123C3]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruxandra Sirbulescu

    Verified customer

    投稿 Dec 08 2011

    Question

    Customer contacted us regarding cross reactivity of ab9272 with hamster nCAM.

    Read More
    Answer


    The ability of ab9272 to recognize the nCAM epitope is dependent on the intact conformation of the first fibronectin type III domain (human nCAM aa506-605). The homology of 93.4% between human (http://www.uniprot.org/uniprot/P13591) and hamster (http://www.ncbi.nlm.nih.gov/protein/XP_003498569.1), including high homology between aa506-605 suggests that cross reactivity is likely. We generally like to see 85% or higher homology before we predict that an antibody will recognize its target in a different species, so the 93.4% homology between human and hamster nCAM is promising.


    As I mentioned, we would be happy to hear whether or not the antibody is able to detect hamster nCAM1 through an AbReview, and you will also receive abPoints that can be used for amazon gift cards or dollars off of a future Abcam purchase. Information about submitting an abReview is provided through the link below:
    https://www.abcam.com/index.html?pageconfig=resource&rid=10332

    Read More

    Question

    Can you tell me the immunogen sequence for this product?

    Read More
    Answer

    Thank you for your enquiry. Unfortunately, no specific immunogen sequence is known, since the antibody was raised against a membrane preparation of SCLC and not against a specific amino acid sequence.

    Read More

    Question

    Rat neuro cell line

    Read More
    Answer

    Thank you for your enquiry. Following correspondence with the lab I have determined that both ab9018 and ab9272 react against all three isoforms of NCAM. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Thank you for your help. I was particularly interested in products, ab8077, ab9272, ab9018 and possibly ab36 although this last one only reacts with human and not rat. The extracellular domains of NCAM 120, 140 and 180 isoforms are identical but NCAM-120 does not have a cytoplasmic domain as it is linked by a GPI anchor to the membrane. 140 and 180 have cytoplasmic domains of differing lengths. So as long as the antibody recognises something on the extracellular domain it should recognise NCAM-120 as well. Best regards,

    Read More
    Answer

    Thank you for your enquiry. Ab8077 recognises NCAM-140 and 180 we do not have any information relating to NCAM 120. I am still in the process of sourcing further information regarding Ab9018 and Ab9272 and will be in touch with you again when I have another answer.

    Read More

    Question

    I am interested in knocking out the expression of N-CAMs in SH-SY5Y human neuroblastoma cells as part of my studies. Therefore i will be requiring an antibody that i can quantify this knock-down with via western blotting. Do you have an antibody that can detect the three major isoforms-i.e. molecular weights of approx 140, 180 and 220 kDa? Or will i need to purchase two or more antibodies?

    Read More
    Answer

    Thank you for your enquiry and for your patience. I took a look at the NCAM antibodies that we currently have, and it does not appear that any of them recognize all three major isoforms. For example, ab8077, clone 123C3, recognizes a transmembrane glycoprotein of 140 and 180 kD and ab8596, clone B332 (123A8), reacts with a 135/220 kD glycosylated and sialyated protein. All the information that we have at this time regarding our antibodies is located on the online datasheets. If you have any more questions regarding them, please contact us again.

    Read More

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