AB250699

Anti-NC2 alpha 抗体 [EPR15426(B)] - BSA and Azide free

Anti-NC2 alpha antibody [EPR15426(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NC2 alpha antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Dr1-associated corepressor, Dr1-associated protein 1, Negative cofactor 2-alpha, NC2-alpha, DRAP1

1 Images

Supplier Data

Western blot - Anti-NC2 alpha antibody [EPR15426(B)] - BSA and Azide free (AB250699)

This data was developed using ab183726, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NC2 alpha antibody [EPR15426(B)] (<a href='/products/primary-antibodies/nc2-alpha-antibody-epr15426b-ab183726'>ab183726</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

SKBR-3 cell lysate at 20 µg

Lane 3:

Human testis cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15426(B)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab250699 is the carrier-free version of ab183726.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NC2 alpha also known as Dr1-associated cofactor 1 (Drap1) is a transcriptional regulatory protein with a molecular mass of approximately 65 kDa. It functions mechanically as a repressor of RNA polymerase II transcription and is widely expressed in various tissues with higher levels noted in the liver and brain. NC2 alpha exerts its function by binding to TBP-DNA complexes modulating their transcriptional activities. Its role as a negative regulatory element is critical in controlling gene expression.

Biological function summary

NC2 alpha participates as part of the NC2 complex partnering with NC2 beta to fine-tune transcription initiation. The complex competitively interacts with TBP influencing the accessibility of transcription factors to the promoter regions. This regulation impacts a broad range of cellular processes which include cell cycle progression and differentiation. NC2 alpha through these biological functions plays a role in maintaining cellular homeostasis ensuring that genes are expressed at appropriate levels.

Pathways

NC2 alpha integrates into transcriptional regulation pathways that are significant in controlling gene expression. Within these pathways NC2 alpha collaborates with proteins such as TBP and NC2 beta which together influence the initiation phase of transcription. Notable pathways include the TFIID and TFIIB-mediated transcription pathways. Through interaction with these proteins NC2 alpha modulates transcriptional initiation contributing to the proper regulation of gene expression.

NC2 alpha associates with several pathological conditions including cancer and neurodegenerative diseases. In cancer the dysregulation of NC2 alpha influences gene expression patterns potentially leading to tumorigenesis. In neurodegenerative diseases NC2 alpha may interfere with transcriptional regulation contributing to the pathological features of these disorders. The interplay between NC2 alpha and TBP in these conditions highlights its significant role in disease progression marking it as a potential target for therapeutic intervention.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.

See full target information DRAP1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NC2 alpha 抗体 [EPR15426(B)] - BSA and Azide free

Anti-NC2 alpha antibody [EPR15426(B)] - BSA and Azide free

Western blot - Anti-NC2 alpha antibody [EPR15426(B)] - BSA and Azide free (AB250699)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise