Anti-Nav1.8/SCN10A 抗体 (ab63331)
Key features and details
- Rabbit polyclonal to Nav1.8/SCN10A
- Suitable for: ICC, WB, IHC-Fr
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Nav1.8/SCN10A antibody
Nav1.8/SCN10A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nav1.8/SCN10A -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-Frmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to Nav1.8/SCN10A aa 1892-1956.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: PBS, Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63331の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
1/4000. Predicted molecular weight: 217 kDa.
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| IHC-Fr |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
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WB
1/4000. Predicted molecular weight: 217 kDa. |
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IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. -
組織特異性
Expressed in the dorsal root ganglia and sciatic nerve. -
関連疾患
Episodic pain syndrome, familial, 2 -
配列類似性
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain. -
ドメイン
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
翻訳後修飾
Ubiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
細胞内局在
Cell membrane. It can be translocated to the cell membrane through association with S100A10. - Information by UniProt
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参照データベース
- Entrez Gene: 29571 Rat
- SwissProt: Q62968 Rat
- Unigene: 10246 Rat
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別名
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
see all
画像
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Western blot - Anti-Nav1.8/SCN10A antibody (ab63331)
Western blot analysis. A single band of strong NaV1.8/SCN10A-like immunoreactivity at approximately 240 kDa in the membrane fraction of rat DRG is shown. Note that membrane fractions of rat whole brain, cerebellum, skeletal muscle, heart, superior cervical ganglia, liver and kidney showed no detectable immunoreactivity. Membrane extract from CHO-SNS22 (a cell line stably transfected with rat NaV1.8/SCN10A cDNA) showed strong immunoreactivity at the same size with DRG. Non-transfected CHO cells did not show any bands.
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Immunohistochemistry (Frozen sections) - Anti-Nav1.8/SCN10A antibody (ab63331)Immunohistochemistry was performed on 2 week old rat lumber DRG sections. NaV1.8/SCN10A-like immunoreactivity can be detected only in small diameter (<30 um) neurons.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab63331 は 3 報の論文で使用されています。
- Latragna A et al. Prdm12 modulates pain-related behavior by remodeling gene expression in mature nociceptors. Pain 163:e927-e941 (2022). PubMed: 34961757
- Desiderio S et al. Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA. Cell Rep 26:3522-3536.e5 (2019). PubMed: 30917309
- Isensee J et al. Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127:216-29 (2014). PubMed: 24190886
