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Anti-Nav1.7 抗体 (ab65167)

Reviews (3)Q&A (2)References (9)

Key features and details

  • Rabbit polyclonal to Nav1.7
  • Suitable for: IHC-Fr, IHC-P, IHC-FoFr, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Nav1.7 antibody
    Nav1.7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Nav1.7

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, IHC-FoFr, ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide within Human Nav1.7 aa 1000-1100. The exact sequence is proprietary.
    (Peptide available as ab192016)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab65167の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21441906
ICC/IF (1)
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21441906
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784).

  • 組織特異性

    Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.

  • 関連疾患

    Primary erythermalgia
    Indifference to pain, congenital, autosomal recessive
    Paroxysmal extreme pain disorder
    Generalized epilepsy with febrile seizures plus 7
    Febrile seizures, familial, 3B

  • 配列類似性

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
    Contains 1 IQ domain.

  • ドメイン

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

  • 翻訳後修飾

    Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
    Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.

  • 細胞内局在

    Cell membrane. Cell projection. In neurite terminals.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ETHA antibody
    • GEFSP7 antibody
    • hNE Na antibody
    • hNE-Na antibody
    • hNENa antibody
    • NE NA antibody
    • NENA antibody
    • Neuroendocrine sodium channel antibody
    • Peripheral sodium channel 1 antibody
    • PN1 antibody
    • Scn9a antibody
    • SCN9A_HUMAN antibody
    • Sodium channel protein type 9 subunit alpha antibody
    • Sodium channel protein type IX subunit alpha antibody
    • Sodium channel voltage gated type IX alpha antibody
    • Sodium channel voltage gated type IX alpha polypeptide antibody
    • Sodium channel voltage gated type IX alpha subunit antibody
    • Voltage gated sodium channel alpha subunit Nav1.7 antibody
    • Voltage gated sodium channel subunit alpha Nav1 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.7 antibody
    see all

参考文献 (9)

ab65167 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab65167 は 9 報の論文で使用されています。

  • Pintacuda G  et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
  • Kwon M  et al. Inhibition of the Nav1.7 Channel in the Trigeminal Ganglion Relieves Pulpitis Inflammatory Pain. Front Pharmacol 12:759730 (2021). PubMed: 34955831
  • Atmaramani RR  et al. Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro. Bioengineering (Basel) 7:N/A (2020). PubMed: 32429423
  • Hulme AJ  et al. Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons. Front Cell Neurosci 14:600895 (2020). PubMed: 33362470
  • Sun W  et al. Inhibition of lncRNA X inactivate-specific transcript ameliorates inflammatory pain by suppressing satellite glial cell activation and inflammation by acting as a sponge of miR-146a to inhibit Nav 1.7. J Cell Biochem 119:9888-9898 (2018). PubMed: 30129228
  • Cai YY  et al. Effects of SCN9A gene modification on Na+ channel and the expression of nerve growth factor in a rat model of diarrhea-predominant irritable bowel syndrome. Mol Med Rep 17:1839-1846 (2018). PubMed: 29138838
  • Rice FL  et al. Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine. Cephalalgia 37:1350-1372 (2017). PubMed: 27852962
  • Henningsen K  et al. Candidate hippocampal biomarkers of susceptibility and resilience to stress in a rat model of depression. Mol Cell Proteomics 11:M111.016428 (2012). IHC-FoFr ; Rat . PubMed: 22311638
  • Weiss J  et al. Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. Nature 472:186-90 (2011). IHC-FoFr ; Mouse . PubMed: 21441906

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nav1.7 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 14 2014

Western blot abreview for Anti-Nav1.7 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (DRG / Brain)
Loading amount
10 µg
Specification
DRG / Brain
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 24 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-Nav1.7 antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification
Dorsal Root Ganglia (DRG) neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - tween 20
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 18 2012

Question

Thank you for your answer.
As we now collaborate with someone who gave us home-made anti-hNav1.7 antibodies, we would appreciate if you can refund.
Tell me how we can proceed.

Best regards,

Read More
Answer

Thank you for contacting us.

Your credit note ID is xx


I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Question

Inquiry: We tested this antibody because it's the only one commercially avalaible which recognized Nav1.7 in human tissues and which is published. But we failed to observe any labelling in our "control" tissues where Nav1.7 is known to be expressed (fibers in dental pulp, Luo et al, 2008 fibers in skin). There is no specific immunolabelling when hNav1.7 is expressed in HEK cells. Find in the attached file more details concerning the different protocols we tested. This is very disappointing.

Read More
Answer

Thank you for taking time to put the information together and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

As a free of charge replacement we can offer you another vial of this same antibody or an alternative antibody. The new vial would obviously also be covered by our Abpromise guarantee. We have currently only the same batch of ab65167in stock. However we have not received any other negative feedback on this batch or on any batch during the last two years.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

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