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リコンビナントRabMAb

Anti-Nav1.6/SCN8A 抗体 [EPR25137-45] - BSA and Azide free (ab302787)

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Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunoprecipitation - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Immunoprecipitation - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
  • Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25137-45] to Nav1.6/SCN8A - BSA and Azide free
  • Suitable for: IP, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Nav1.6/SCN8A antibody [EPR25137-45] - BSA and Azide free
    Nav1.6/SCN8A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25137-45] to Nav1.6/SCN8A - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, IHC-P, WB, IHC-Frmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse brain tissue lysate. Rat spinal cord tissue lysate. Human cerebellum and spinal cord tissue lysate. HEK-293T cells transfected with a his tagged Human SCN8A expression vector, whole cell lysate. IHC-P: Mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat frozen cerebrum tissue (fresh). IP: Mouse and rat brain tissue lysate.

  • 特記事項

    ab302787 is a carrier free version of ab302776. 

    ab302786 does not react in: IHC-P with human samples; ICC/IF with human, mouse, and rat species; intracellular flow cytometry with mouse and rat samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302787の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 260 kDa (predicted molecular weight: 225 kDa).
IHC-Fr
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 260 kDa (predicted molecular weight: 225 kDa).
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

  • 機能

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.

  • 組織特異性

    Isoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages.

  • 配列類似性

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.
    Contains 1 IQ domain.

  • ドメイン

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

  • 翻訳後修飾

    May be ubiquitinated by NEDD4L; which would promote its endocytosis.

  • 細胞内局在

    Membrane and Cytoplasmic vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CerIII antibody
    • CIAT antibody
    • EIEE13 antibody
    • hNa6/Scn8a voltage gated sodium channel antibody
    • MED antibody
    • Motor endplate disease antibody
    • NaCh 6 antibody
    • NaCh6 antibody
    • Nav 1.6 antibody
    • Nbna1 antibody
    • peripheral nerve protein type 4 antibody
    • PN 4 antibody
    • PN4 antibody
    • SCN8A antibody
    • SCN8A_HUMAN antibody
    • Sodium channel protein type 8 alpha subunit antibody
    • Sodium channel protein type 8 subunit alpha antibody
    • Sodium channel protein type VIII alpha subunit antibody
    • Sodium channel protein type VIII subunit alpha antibody
    • Sodium channel voltage gated type VIII alpha antibody
    • Sodium channel voltage gated type VIII alpha polypeptide antibody
    • Sodium channel voltage gated type VIII alpha subunit antibody
    • Voltage gated sodium channel subunit alpha Nav1.6 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.6 antibody
    see all

画像

  • Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    All lanes : Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Rat liver tissue lysate
    Lane 5 : Human spinal cord tissue lysate
    Lane 6 : Human cerebellum tissue lysate
    Lane 7 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 225 kDa
    Observed band size: 260 kDa why is the actual band size different from the predicted?



    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 26410685).

    Negative control: Liver (PMID: 11532991).

    Bands around 150 kDa and 250 kDa may due to degradation.

    Samples are non-boiled as boiling may cause protein aggregates.
    Lane 5-7 of the blot was developed using a high sensitivity ECL substrate. 

    Exposure time: Lane 1-4: 114 seconds, Lane 5-7: 48 seconds.

  • Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    All lanes : Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786) at 1/1000 dilution

    Lane 1 : HEK-293T cells transfected with a Human SCN8A expression vector containing a his tag, whole cell lysate
    Lane 2 : HEK-293T cells transfected with a Human SCN2A expression vector containing a his tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Performed under non-reducing conditions.

    Predicted band size: 225 kDa



    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.
    This antibody does not cross-react with human SCN2A.

    Exposure time: 3.25 seconds. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 dilution (0.246 µg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on axon in mouse cerebrum. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin.

    Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 dilution (0.246 µg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on axon in rat cerebrum. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin.

    Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 dilution (0.246 µg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin.

    Negative control: No staining on mouse liver is observed. 

    Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 dilution (0.246 µg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin.

    Negative control: No staining on rat liver is observed. 

    Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 dilution (0.982 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml) (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml). 

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 dilution (0.982 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: no staining on mouse liver is observed (PMID: 11532991). 

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution. 

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

    Positive staining on rat cerebrum is observed.

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution. 

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: no staining on rat liver is observed (PMID: 11532991).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution. 

  • Immunoprecipitation - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunoprecipitation - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Nav1.6/SCN8A was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab302786 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302786 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: mouse brain tissue lysate 10 µg (Input)

    Lane 2: ab302786 IP in mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302786 in mouse brain tissue lysate

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    Observed MW (kDa): 260

    Input is non-boiled as boiling may cause protein aggregates.

  • Immunoprecipitation - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Immunoprecipitation - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

    This data was developed using ab302786, the same antibody clone in a different buffer formulation.

    Nav1.6/SCN8A was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab302786 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302786 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: rat brain tissue lysate 10 µg (Input)

    Lane 2: ab302786 IP in rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302786 in rat brain tissue lysate

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    Observed MW (kDa): 260 

    Input is non-boiled as boiling may cause protein aggregates.

  • Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)
    Anti-Nav1.6/SCN8A antibody [EPR25137-45] (BSA and Azide free) (AB302787)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302787 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302787 は論文での使用が確認できていません。

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