AB303657

Anti-Nav1.5/SCN5A 抗体 [N397/19]

Anti-Nav1.5/SCN5A antibody [N397/19]

Recombinant
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Mouse Recombinant Monoclonal SCN5A antibody. Suitable for WB and reacts with Mouse, Rat samples.

別名を表示する

Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, hH1, SCN5A

1 Images

Supplier Data

Western blot - Anti-Nav1.5/SCN5A antibody [N397/19] (AB303657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen (PMID : 1309946). Samples are non-boiled as boiling may cause protein aggregates. This blot was developed using a higher sensitivity ECL substrate. Exposure time : 169 minutes

All lanes:

Western blot - Anti-Nav1.5/SCN5A antibody [N397/19] (ab303657) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 40 µg

Lane 2:

Mouse spleen tissue lysate at 40 µg

Lane 3:

Rat heart tissue lysate at 40 µg

Lane 4:

Rat spleen tissue lysate at 40 µg

Secondary

All lanes:

Western blot at 1/5000 dilution

Observed band size: 260 kDa

true

Exposure time: 169min

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

N397/19

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Rat

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nav1.5 also known as SCN5A is a protein that functions mechanically as a voltage-gated sodium channel. This channel facilitates the rapid influx of sodium ions across the cell membrane leading to the depolarization necessary for action potential initiation and propagation in cardiac cells. Nav1.5 weighs approximately 220-260 kDa and is primarily expressed in cardiac myocytes where it plays a central role in maintaining cardiac excitability and conduction.

Biological function summary

The sodium channel Nav1.5 is integral to the electrical signaling within the heart. It functions within the cardiac cell membrane and forms part of the larger sodium channel complex. Nav1.5 maintains ionic balance and supports normal cardiac rhythm making it essential for the contraction of the heart. Its expression and function can be influenced by interacting proteins and intracellular signaling molecules.

Pathways

Nav1.5 is an important component in the cardiac action potential generation and conduction pathways. It directly influences the cardiac conduction system and is part of the pathway that encompasses the sodium-potassium pump and other ion channels like Nav1.1 and Nav1.8. Through these pathways Nav1.5 regulates cardiac rhythm and myocardial contractility ensuring efficient heart function.

Nav1.5 mutations have significant associations with arrhythmias and cardiac conduction issues such as long QT syndrome and Brugada syndrome. These cardiac conditions are linked to disrupted sodium channel function caused by mutations in the SCN5A gene. Nav1.1 and Nav1.2 other family members of the sodium channel can sometimes show altered expression in these disorders reflecting the interconnected nature of this protein family in cardiac health.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Pore-forming subunit of Nav1.5, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 1309946, PubMed : 21447824, PubMed : 23085483, PubMed : 23420830, PubMed : 25370050, PubMed : 26279430, PubMed : 26392562, PubMed : 26776555). Nav1.5 is the predominant sodium channel expressed in myocardial cells and it is responsible for the initial upstroke of the action potential in cardiac myocytes, thereby initiating the heartbeat (PubMed : 11234013, PubMed : 11804990, PubMed : 12569159, PubMed : 1309946). Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2 (By similarity).

See full target information SCN5A

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nav1.5/SCN5A 抗体 [N397/19]

Anti-Nav1.5/SCN5A antibody [N397/19]

Western blot - Anti-Nav1.5/SCN5A antibody [N397/19] (AB303657)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise