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Anti-Natural Killer Cell Activation Structures 抗体 [ANK61] (ab36392)

Submit a review Q&A (4)References (6)

Key features and details

  • Mouse monoclonal [ANK61] to Natural Killer Cell Activation Structures
  • Suitable for: IP, IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Rat
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Natural Killer Cell Activation Structures antibody [ANK61]

  • 製品の詳細

    Mouse monoclonal [ANK61] to Natural Killer Cell Activation Structures

  • 由来種

    Mouse

  • 特異性

    The antigen recognized by ab36392 is highly expressed on freshly isolated and cultured NK cells. The antibody also binds to rat alpha beta TCR T cells and at a low level to B cells.

  • アプリケーション

    適用あり: IP, IHC-Fr, Flow Cyt, IHC-Pmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Tissue, cells or virus corresponding to Rat Natural Killer Cell Activation Structures. Preparation of IL 2 activated cultured NK cells (Wag rats)

  • 特記事項


    Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • 一次抗体 備考

    Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.

  • ポリ/モノ

    モノクローナル

  • クローン名

    ANK61

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab36392の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Natural killer cells (NK) are a type of lymphocyte and a component of innate immune defense. They share a common progenitor with T cells and have been described as large, granular, bone-marrow derived lymphocytes. These cells do not destroy the attacking microorganisms directly; they attack infected cells and cells that appear that they might be cancer. NK cells are not phagocytic; they weaken the target cell's plasma membrane, causing water and ions to diffuse into the cell and expanding it. Under this large pressure, the target cell lyses. NK cells are characterised immunohistochemically by the presence of CD56 and the absence of CD3 on the cell membrane. NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain virus infections while the adaptive immune response is generating antigen specific cytotoxic T cells that can clear the infection. Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection.

参考文献 (6)

ab36392 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab36392 は 6 報の論文で使用されています。

  • Travis OK  et al. Interleukin-17 signaling mediates cytolytic natural killer cell activation in response to placental ischemia. Am J Physiol Regul Integr Comp Physiol 318:R1036-R1046 (2020). PubMed: 32320265
  • Travis OK  et al. Chronic infusion of interleukin-17 promotes hypertension, activation of cytolytic natural killer cells, and vascular dysfunction in pregnant rats. Physiol Rep 7:e14038 (2019). PubMed: 30963715
  • Cottrell JN  et al. Interleukin-4 supplementation improves the pathophysiology of hypertension in response to placental ischemia in RUPP rats. Am J Physiol Regul Integr Comp Physiol 316:R165-R171 (2019). PubMed: 30624978
  • Reyes L  et al. Porphyromonas gingivalis and adverse pregnancy outcome. J Oral Microbiol 10:1374153 (2018). PubMed: 29291034
  • Shields CA  et al. Placental ischemia-stimulated T-helper 17 cells induce preeclampsia-associated cytolytic natural killer cells during pregnancy. Am J Physiol Regul Integr Comp Physiol 315:R336-R343 (2018). PubMed: 29718698
  • Bastidas J  et al. Human Schwann cells exhibit long-term cell survival, are not tumorigenic and promote repair when transplanted into the contused spinal cord. Glia 65:1278-1301 (2017). PubMed: 28543541

レビューと Q&A

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レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Question

One doubt: some of this antibody only react with mouse, and you suggested human tissue. Is it correct?
Thanks,

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Answer

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Question

Our customer would like to know which are the best positive control
and negative control for the antibodies below.
1. anti Natural killer - ab36392
2. anti- interferon gama - ab9918
3. anti TNF alfa - ab34674
4. anti TGF beta - ab15537
5. anti neutrófilo - ab2557
6. anti CD20 - ab9475
7. anti células T CD8 - ab22378
8. anti células T CD4 - ab51312
She will do ICC and IHC-P assays.
Thanks and best regards,

Read More
Answer

Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections

ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

ab2557; mouse spleen tissue sections

ab9475; human tonsil tissue sections

ab22378; mouse spleen tissue sections

ab51312; Thymus tissue sections, human pancreas tissue sections.

The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hello



The customer regarding protocol provide us the datasheet of ab2557, where it is write that the antibody must be stored at 4oC, not at -20oC. It is attached below.



In addition, the customer said that, despite she had used mouse tissue, she did not understand the multiple unespecific dots at both techniques IHC and ICC.

Read More
Answer

Thank you for your reply.

It does look like we changed the storage instructions of ab2557. Since this product was tested in a guaranteed species and technique and she wasn't seeing any staining, we could offer a free of charge replacement order from the original order. Please let me know if that is what the customer would prefer.

For the spotty staining in IHC, it may be that the antibody is binding to the blocking buffer. She should filter the blocking buffer before adding to the slide. Also she should run a no primary control to determine if the secondary is binding to something non-specifically in the slide. Also the tissue maynot washed enough orfixative may still present. And though they used a gentle permeabilization agent, pemeabilization may have damaged the membrane and removed the membrane protein
Remove permeabilizing agent from your buffers.

I hope this information helps. Please let me know what the customer would like to do with the ab2557.

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Question


A customer had some problems with some antibodies that she had bought.
I attached below the questionnarie.
Any doubts, please contact me again,
Best regards,

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Answer

Thank you forhaving your customercomplete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results for them. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

There are some issues with the antibodies that may be resulting in the lack of staining. Firstly, the neutrophil antibody ab2557 was stored in the refrigerator. The datasheet for this antibody says to store at -20C. The last order I have from you for this product is in January. It is possible that storing this antibody improperly for 4 months has affected its quality.

Secondly, the NK antibody ab36392 is not tested in mouse, only rat. We do not guarantee this antibody to work in mouse. Does the customer have access to rat tissue to run a positive control? Also this is the antibody that was giving spotty staining. Have they run a no-primary control? Or tried filtering the blocking buffer?

Lastly, the interferon gamma antibody ab9918 is not tested in IHC-P or ICC and is therefore not guaranteed to work in these applications.

Since two of the antibodies are being used in untested ways and the neutrophil antibody was improperly stored, there are many reasons why the customer may not be seeing staining.

I hope this information is helpful, and I thank you for your cooperation.

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