AB181169

Anti-NASP 抗体 [EPR12827]

Anti-NASP antibody [EPR12827]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal NASP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

別名を表示する

Nuclear autoantigenic sperm protein, NASP

4 Images

Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] (AB181169)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] (AB181169)

Intracellular Flow Cytometry analysis of HeLa cells using ab181169at a 1/40 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/40 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (AB181169)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (AB181169)

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

Supplier Data

Immunoprecipitation - Anti-NASP antibody [EPR12827] (AB181169)

Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NASP antibody [EPR12827] (ab181169)

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-NASP antibody [EPR12827] (AB181169)

160KDa : tNASP
62KDa : sNASP

All lanes:

Western blot - Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Caco-2 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

NTERA2/D1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 85 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12827

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NASP also known as Nuclear Autoantigenic Sperm Protein functions as a histone chaperone. With a molecular mass of approximately 75 kDa NASP assists in the proper assembly and disassembly of nucleosome structures by binding to histones H1 and H2A-H2B. This protein mainly expresses in rapidly dividing tissues especially in the testes but also presents in the thymus and bone marrow. NASP maintains the supply of free histones aiding in DNA replication and transcription processes.

Biological function summary

NASP plays an essential role in chromatin organization and dynamics during the cell cycle. As part of the histone transport complex it stabilizes histones and prevents their aggregation. NASP facilitates proper chromatin assembly necessary for DNA packaging and regulation of gene expression. This activity ensures the accurate transmission of genetic information during cell division impacting processes essential for cell proliferation and function.

Pathways

NASP operates within the chromatin assembly and nucleosome remodeling pathways. It coordinates with other chaperones like NAP1 in chromatin assembly factor-1 (CAF-1) complex. These pathways are integral to DNA replication and repair directly linking NASP to cellular processes critical for maintaining genome integrity. The interaction with importin beta further indicates NASP's role in nuclear import pathways emphasizing its involvement in both nuclear organization and cellular regulation.

NASP associates with cancer and infertility. The aberrant expression of NASP is observed in various tumors implying its contribution to tumorigenesis and proliferation. Its association with other histone chaperones such as ASF1 highlights its potential role in oncogenic pathways. Furthermore alterations in NASP expression affect sperm development linking it to male infertility and emphasizing its necessity in reproductive biology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the histone chaperone network (PubMed : 22195965). Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy (PubMed : 22195965). Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.. Isoform 1. Stabilizes soluble histone H3-H4.. Isoform 2. Stabilizes soluble histone H3-H4.

See full target information NASP

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 32:11 PubMed39838427

2025

The endoplasmic reticulum degradation-enhancing α-mannosidase-like protein 3 attenuates the unfolded protein response and has pro-survival and pro-viral roles in hepatoma cells and hepatocellular carcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Alina-Veronica Ghionescu,Mihaela Uta,Andrei Sorop,Catalin Lazar,Petruta R Flintoaca-Alexandru,Gabriela Chiritoiu,Livia Sima,Stefana-Maria Petrescu,Simona Olimpia Dima,Norica Branza-Nichita

International journal of molecular sciences 25: PubMed39409021

2024

Unraveling the Role of JMJD1B in Genome Stability and the Malignancy of Melanomas.

Applications

Unspecified application

Species

Unspecified reactive species

Perla Cruz,Diego Peña-Lopez,Diego Figueroa,Isidora Riobó,Vincenzo Benedetti,Francisco Saavedra,Claudia Espinoza-Arratia,Thelma M Escobar,Alvaro Lladser,Alejandra Loyola

Frontiers in pharmacology 14:1271435 PubMed38026973

2023

Cathepsin V regulates cell cycle progression and histone stability in the nucleus of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Naphannop Sereesongsaeng,James F Burrows,Christopher J Scott,Klaudia Brix,Roberta E Burden

iScience 26:107042 PubMed37360696

2023

SRSF10 regulates proliferation of neural progenitor cells and affects neurogenesis in developing mouse neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Li,Hanyang Jiang,Yawei Mu,Zixuan Wei,Ankangzhi Ma,Menghan Sun,Jingjing Zhao,Cuiqing Zhu,Xianhua Chen

Molecular cell 83:1075-1092.e9 PubMed36868228

2023

DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo Carraro,Ivo A Hendriks,Colin M Hammond,Victor Solis-Mezarino,Moritz Völker-Albert,Jonas D Elsborg,Melanie B Weisser,Christos Spanos,Guillermo Montoya,Juri Rappsilber,Axel Imhof,Michael L Nielsen,Anja Groth

Nucleic acids research 50:5349-5368 PubMed35489058

2022

NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Bao,Massimo Carraro,Valentin Flury,Yanhong Liu,Min Luo,Liu Chen,Anja Groth,Hongda Huang

Oncology reports 46: PubMed34633056

2021

Knockdown of long noncoding RNA colorectal neoplasia differentially expressed inhibits hepatocellular carcinoma progression by mediating the expression of nuclear autoantigenic sperm protein.

Applications

Unspecified application

Species

Unspecified reactive species

Yungang Zhu,Baoguo Li,Guoping Xu,Changrui Han,Gang Xing

Bioscience, biotechnology, and biochemistry 84:703-713 PubMed31797734

2019

MiR-381-3p suppresses biological characteristics of cancer in head-neck squamous cell carcinoma cells by targeting nuclear autoantigenic sperm protein (NASP).

Applications

Unspecified application

Species

Unspecified reactive species

Fanyong Kong,Lianhe Li,Chaoshan Wang,Qiang Zhang,Shizhi He

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NASP 抗体 [EPR12827]

Anti-NASP antibody [EPR12827]

Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] (AB181169)

Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (AB181169)

Immunoprecipitation - Anti-NASP antibody [EPR12827] (AB181169)

Western blot - Anti-NASP antibody [EPR12827] (AB181169)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

Abcam product promise