Anti-NARG1 抗体 [434C2a] (ab60065)
![Western blot - Anti-NARG1 antibody [434C2a] (ab60065)](https://www.abcam.co.jp/ps/products/60/ab60065/Images/ab60065_WB_1.jpg "Western blot - Anti-NARG1 antibody [434C2a] (ab60065)")
Key features and details
- Mouse monoclonal [434C2a] to NARG1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
-
製品名
Anti-NARG1 antibody [434C2a]
NARG1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [434C2a] to NARG1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment (Human)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified using protein G column chromatography, from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
434C2a -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60065の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa for the recombinant protein fragment (predicted molecular weight: 101 kDa for the full length protein).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa for the recombinant protein fragment (predicted molecular weight: 101 kDa for the full length protein).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
機能
The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter. -
組織特異性
Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. -
配列類似性
Contains 8 TPR repeats. -
翻訳後修飾
Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment. -
細胞内局在
Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes. - Information by UniProt
-
別名
- 5730450D16Rik antibody
- 6330400I15 antibody
- ASTBDN antibody
see all
画像
-
Western blot - Anti-NARG1 antibody [434C2a] (ab60065)Western blot analysis of immunized recombinant protein, using ab60065
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab60065 は 3 報の論文で使用されています。
- Cirinelli A et al. Evidence that the transcriptional repressor ICER is regulated via the N-end rule for ubiquitination. Exp Cell Res 414:113083 (2022). PubMed: 35227662
- Kweon HY et al. Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway. Elife 10:N/A (2021). PubMed: 34355692
- Dörfel MJ et al. Proteomic and genomic characterization of a yeast model for Ogden syndrome. Yeast 34:19-37 (2017). WB . PubMed: 27668839
