AB211529

Anti-NAPRT1 抗体 [CL0665]

Name: Anti-NAPRT1 抗体 [CL0665] (ab211529) | Abcam
Brand: Abcam Limited
SKU: AB211529
Price: 70000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-NAPRT1 antibody [CL0665]

(1 Review)

(5 Publications)

Mouse Monoclonal NAPRT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 50 to C-terminus.

別名を表示する

FHIP, NAPRT1, NAPRT, Nicotinate phosphoribosyltransferase, NAPRTase, FHA-HIT-interacting protein, Nicotinate phosphoribosyltransferase domain-containing protein 1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemical analysis of paraffin-embedded Humanbreast cancer tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CL0665

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human NAPRT aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6XQN6

エピトープ

Binds to an epitope located within the peptide sequence PAFFEHLRALDCSEV (aa90-104) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NAPRT1 also known as nicotinate phosphoribosyltransferase plays an important role in the Preiss-Handler pathway where it converts nicotinic acid (a form of vitamin B3) to nicotinic acid mononucleotide. NAPRT1 is approximately 55 kDa in size. This protein mainly expresses in liver kidney and several tissues with high metabolic rates. An accurate understanding of NAPRT1’s expression is essential for targeting its function in NAD+ biosynthesis.

Biological function summary

Nicotinate phosphoribosyltransferase participates in the biosynthesis of NAD+ an important cofactor in redox reactions. It combines with other enzymes to effectively maintain NAD+ levels in cells. NAD+ supports cellular metabolism DNA repair and longevity. NAPRT1's activity integrated with other components sustains cellular energy processes and influences cellular health.

Pathways

NAD+ biosynthesis involves multiple mechanisms where NAPRT1 holds importance. One significant pathway includes the Preiss-Handler pathway where NAPRT1 works in tandem with NMNAT1 to ensure NAD+ production. Other pathways also connect NAPRT1 with NAMPT another enzyme that converts nicotinamide to NAD+ highlighting the biochemical network sustaining NAD+ levels important for cell function.

Alterations in NAPRT1 expression relate to pellagra a condition resulting from vitamin B3 deficiency. Research also links NAPRT1 to certain cancers where its expression may support tumor metabolism. NAPRT1's association with NAMPT makes it a potential biomarker for therapeutic strategies aiming to modulate NAD+ biosynthesis in pathological states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed : 17604275, PubMed : 21742010, PubMed : 26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed : 17604275).

See full target information NAPRT

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:342 PubMed40280967

2025

Targeting metabolic vulnerability by combining NAMPT inhibitors and disulfiram for treatment of recurrent ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kei Kudo,Yoshimi Endo Greer,Daniel R Crooks,Ye Yang,Jeffrey R Brender,Teruhiko Yoshida,Brittney S Harrington,Rahul Kamdar,Soumya Korrapati,Yusuke Shibuya,Leah Henegar,Jeffrey Kopp,Takeo Fujii,Stanley Lipkowitz,Christina M Annunziata

iScience 28:112283 PubMed40241769

2025

Exploiting the therapeutic vulnerability of IDH-mutant gliomas with zotiraciclib.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Pang,Qi Li,Zach Sergi,Guangyang Yu,Olga Kim,Peng Lu,Marina Chan,Xueyu Sang,Herui Wang,Alice Ranjan,Robert W Robey,Ferri Soheilian,Bao Tran,Felipe J Núñez,Meili Zhang,Hua Song,Wei Zhang,Dionne Davis,Mark R Gilbert,Michael M Gottesman,Zhenggang Liu,Craig J Thomas,Maria G Castro,Taranjit S Gujral,Jing Wu

Neoplasia (New York, N.Y.) 41:100903 PubMed37148658

2023

Mitochondrial rewiring drives metabolic adaptation to NAD(H) shortage in triple negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Agata Sofia Assuncao Carreira,Silvia Ravera,Chiara Zucal,Natthakan Thongon,Irene Caffa,Cecilia Astigiano,Nadia Bertola,Arianna Buongiorno,Michela Roccuzzo,Alessandra Bisio,Barbara Pardini,Alessio Nencioni,Santina Bruzzone,Alessandro Provenzani

Cancer & metabolism 6:1 PubMed29541451

2018

Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA.

Applications

Unspecified application

Species

Unspecified reactive species

Natthakan Thongon,Chiara Zucal,Vito Giuseppe D'Agostino,Toma Tebaldi,Silvia Ravera,Federica Zamporlini,Francesco Piacente,Ruxanda Moschoi,Nadia Raffaelli,Alessandro Quattrone,Alessio Nencioni,Jean-Francois Peyron,Alessandro Provenzani

Cancer research 77:3857-3869 PubMed28507103

2017

Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Piacente,Irene Caffa,Silvia Ravera,Giovanna Sociali,Mario Passalacqua,Valerio G Vellone,Pamela Becherini,Daniele Reverberi,Fiammetta Monacelli,Alberto Ballestrero,Patrizio Odetti,Antonia Cagnetta,Michele Cea,Aimable Nahimana,Michel Duchosal,Santina Bruzzone,Alessio Nencioni

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NAPRT1 抗体 [CL0665]

Anti-NAPRT1 antibody [CL0665]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise