Anti-NANOS2 抗体 (ab70000)
Key features and details
- Rabbit polyclonal to NANOS2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NANOS2 antibody -
製品の詳細
Rabbit polyclonal to NANOS2 -
由来種
Rabbit -
特異性
ab70000 does not cross-react with either Nanos1 or Nanos3. -
アプリケーション
適用あり: WBmore details
適用なし: IHC -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human NANOS2 (C terminal). 13 amino acid peptide near the carboxy terminus of the human NANOS2.
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ポジティブ･コントロール
- WB: SK-N-SH cell lysate
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特記事項
Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
ターゲット情報
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機能
Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state. -
組織特異性
Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level). -
配列類似性
Belongs to the nanos family.
Contains 1 nanos-type zinc finger. -
発生段階
Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level). -
ドメイン
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. -
細胞内局在
Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis. - Information by UniProt
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参照データベース
- Entrez Gene: 339345 Human
- Entrez Gene: 365213 Rat
- Omim: 608228 Human
- SwissProt: P60321 Human
- Unigene: 434218 Human
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別名
- NANO2_HUMAN antibody
- Nanos homolog 2 antibody
- Nanos homolog 2 Drosophila antibody
see all
画像
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Western blot - Anti-NANOS2 antibody (ab70000)Anti-NANOS2 antibody (ab70000) at 2 µg/ml + SK-N-SH cell lysate at 15 µg
Predicted band size: 15 kDa
Observed band size: 15 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab70000 は 4 報の論文で使用されています。
- Lei JH et al. Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway. World J Stem Cells 12:500-513 (2020). PubMed: 32742567
- Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells. J Cell Biochem 120:1174-1184 (2019). PubMed: 30335884
- Kong D et al. PKA regulatory IIa subunit is essential for PGD2-mediated resolution of inflammation. J Exp Med 213:2209-26 (2016). PubMed: 27621415
- Kusz KM et al. The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction. Mol Hum Reprod 15:165-71 (2009). WB, IHC-P ; Human . PubMed: 19168545