Anti-Nanog 抗体 [NNG-811] (ab62734)
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)](https://www.abcam.co.jp/ps/products/62/ab62734/Images/ab62734-76123-anti-nanog-antibody-nng-811-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)")
Key features and details
- Mouse monoclonal [NNG-811] to Nanog
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Nanog antibody [NNG-811]
Nanog 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [NNG-811] to Nanog -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length human Nanog.
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ポジティブ･コントロール
- Extracts of NT2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
Purified Immunoglobulin -
ポリ/モノ
モノクローナル -
クローン名
NNG-811 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62734の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
組織特異性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
配列類似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
発生段階
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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別名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)
3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 µg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
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![Western blot - Anti-Nanog antibody [NNG-811] (ab62734)](https://www.abcam.co.jp/ps/products/62/ab62734/Images/ab62734-76122-anti-nanog-antibody-nng-811-western-blot.jpg)
Western blot - Anti-Nanog antibody [NNG-811] (ab62734)Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
Lane 3 : Negative control – no primary antibody
All lanes : Cell extract of NT2 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)](https://www.abcam.co.jp/ps/products/62/ab62734/Images/ab62734-297639-anti-nanog-antibody-nng-811-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)Nanog was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract using ab62734.
Lane 1: 5 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 2: 10 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 3: Negative control without cell extract.
Lane 4: NT2/D1 whole cell extract (input).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (44)
ab62734 は 44 報の論文で使用されています。
- Rizzo G et al. Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn's Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition. Cell Mol Gastroenterol Hepatol 15:741-764 (2023). PubMed: 36521659
- Sasserath T et al. An induced pluripotent stem cell-derived NMJ platform for study of the NGLY1-Congenital Disorder of Deglycosylation. Adv Ther (Weinh) 5:N/A (2022). PubMed: 36589922
- Lawrence ML et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity. iScience 25:103884 (2022). PubMed: 35243244
- Oldershaw RA et al. Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype. Biomedicines 10:N/A (2022). PubMed: 36551899
- Shapira E et al. High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction. Ann Plast Surg 89:e60-e68 (2022). PubMed: 36416705