Anti-Nanog 抗体 (ab21624)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: ICC/IF, WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Nanog antibody
Nanog 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nanog -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, Sandwich ELISAmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human Nanog aa 29-49.
Sequence:CGPEENYPSLQMSSAEMPHTE
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特記事項
Concentration of Anti-Nanog antibody: 0.2 mg/mL
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21624の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (10) |
1/1000.
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WB | (3) |
1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
Sandwich ELISA |
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.
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特記事項 |
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ICC/IF
1/1000. |
WB
1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture. |
ターゲット情報
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機能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
組織特異性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
配列類似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
発生段階
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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別名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
画像
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ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
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All lanes : Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1 : Human fetal fibroblast lysate
Lane 2 : Human iPS cell lysate
Predicted band size: 34 kDa -
All lanes : Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot. -
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624)Image courtesy of Mick Wauchope
Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
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Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (282)
ab21624 は 282 報の論文で使用されています。
- Feng X et al. Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy. Brain 146:278-294 (2023). PubMed: 35867854
- He Y et al. 4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis. Protein Cell 14:202-216 (2023). PubMed: 36929036
- Akter M et al. Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene. Stem Cell Res 69:103078 (2023). PubMed: 36965406
- Yang J et al. TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways. Environ Toxicol 38:2002-2010 (2023). PubMed: 37219039
- Liu X et al. Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa. Elife 12:N/A (2023). PubMed: 37272616