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リコンビナントRabMAb

Anti-NAK/TBK1 (phospho S172) 抗体 [EPR2867(2)] - BSA and Azide free (ab186469)

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Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
  • Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free
    NAK/TBK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    Stimulation may be required to allow detection of the phosphorylated protein.  Please see images below for recommended treatment conditions and positive controls.

  • アプリケーション

    適用あり: Dot blot, WBmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab206124)

  • 特記事項

    ab186469 is the carrier-free version of ab109272.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186469の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124).
特記事項
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124).
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.

  • 組織特異性

    Ubiquitous with higher expression in testis.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EC 2.7.11.1 antibody
      • FLJ11330 antibody
      • FTDALS4 antibody
      • NAK antibody
      • NF kappa B activating kinase antibody
      • NF kB activating kinase antibody
      • NF-kappa-B-activating kinase antibody
      • Serine/threonine protein kinase TBK 1 antibody
      • Serine/threonine protein kinase TBK1 antibody
      • Serine/threonine-protein kinase TBK1 antibody
      • T2K antibody
      • TANK binding kinase 1 antibody
      • TANK-binding kinase 1 antibody
      • TBK 1 antibody
      • Tbk1 antibody
      • TBK1_HUMAN antibody
      see all

    画像

    • Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
      Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

      Dot blot analysis of NAK/TBK1 (phospho S172) phospho peptide (Lane 1) and NAK/TBK1 non-phospho peptide (Lane 2) labelling NAK/TBK1 (phospho S172) phospho peptide with ab109272 at a dilution of 1:1000 dilution (0.627μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

      Blocking buffer: 5% NFDM/TBST.
      Dilution buffer: 5% NFDM /TBST .

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).

    • Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
      Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab186469 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186469 は 1 報の論文で使用されています。

    • Han X  et al. Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway. Open Med (Wars) 16:581-590 (2021). PubMed: 33869780

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