Anti-NAK/TBK1 (phospho S172) 抗体 [EPR2867(2)] (ab109272)

Thank you for your recent telephone call. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

It has taken a little time to obtain more information regarding the WB testing protocol and I appreciate your patience. I can confirm this particular product is sourced and tested externally, so we regrettably have slightly limited information in this case. But they have forwarded the following:

Nitrocellulose membrane blocked with 5% milk and did not observe any background. The primary antibody dilution in the image was 1:10,000.

In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

As discussed on the telephone, there is not much more to suggest in the case and I am sorry to suggest you may have regrettably received a bad vial on this occasion. I would be pleased to arrange a free of charge replacement or credit note.

In addition, in order to otain more information for our quality records, I would aprpeciate if you could complete the following questionnaire which we discussed on the telephone. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire and details of how you would like to proceed.

Order Details Antibody code:
Problem Choose: Non-specific band Multiple bands No signal or weak signal High background
Lot number
Purchase order number or preferably Abcam order number:
General Information Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, wrong band size, more bands, no band etc.)
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
Amount of protein loaded
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Detection method (ECL, ECLPlus etc.)
Positive and negative controls used (please specify)
Optimization attempts (problem solving) How many times have you tried the Western?
Have you run a "No Primary" control? Yes No
Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place?
What steps have you altered?
Additional Notes:
Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.