Anti-NAIP 抗体 (ab25968)
Key features and details
- Rabbit polyclonal to NAIP
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NAIP antibody
NAIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NAIP -
由来種
Rabbit -
特異性
ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP). -
アプリケーション
適用あり: WB, ELISA, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25968の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 20844241
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 20844241 |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. -
組織特異性
Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord. -
配列類似性
Contains 3 BIR repeats.
Contains 1 NACHT domain. - Information by UniProt
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参照データベース
- Entrez Gene: 100510692 Human
- Entrez Gene: 4671 Human
- Omim: 600355 Human
- SwissProt: Q13075 Human
- Unigene: 654500 Human
- Unigene: 710305 Human
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別名
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
see all
画像
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Western Blot of human tonsil lysate labeling NAIP with Anti-NAIP antibody (ab25968) at 1µg/ml.
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Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms. -
ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF
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Western blot - Anti-NAIP antibody (ab25968)This image is courtesy of an Abreview submitted by Dr Mahesh ShivananjappaAnti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg
Secondary
HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa (possible non-specific binding)
Exposure time: 1 minute
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Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab25968 は 12 報の論文で使用されています。
- Wu J et al. Changes in expression levels of Nod-like receptors in the spleen of ewes. Anim Reprod 20:e20220093 (2023). PubMed: 37228386
- Zhao Z et al. Early Pregnancy Modulates Expression of the Nod-like Receptor Family in Lymph Nodes of Ewes. Animals (Basel) 12:N/A (2022). PubMed: 36496806
- Zhang L et al. Modulation of Nod-like Receptor Expression in the Thymus during Early Pregnancy in Ewes. Vaccines (Basel) 10:N/A (2022). PubMed: 36560538
- Triantafilou K et al. Differential recognition of HIV-stimulated IL-1ß and IL-18 secretion through NLR and NAIP signalling in monocyte-derived macrophages. PLoS Pathog 17:e1009417 (2021). PubMed: 33861800
- Huang Z et al. Inhibition of caspase-3-mediated GSDME-derived pyroptosis aids in noncancerous tissue protection of squamous cell carcinoma patients during cisplatin-based chemotherapy. Am J Cancer Res 10:4287-4307 (2020). PubMed: 33415000