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リコンビナントRabMAb

Anti-NAF1 抗体 [EPR11516] (ab171078)

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Western blot - Anti-NAF1 antibody [EPR11516] (ab171078)
  • Flow Cytometry (Intracellular) - Anti-NAF1 antibody [EPR11516] (ab171078)
  • Anti-NAF1 antibody [EPR11516] (ab171078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11516] to NAF1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NAF1 antibody [EPR11516]
    NAF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11516] to NAF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, A431, A549, Namalwa and Caco-2 cell lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab171078の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.

  • 配列類似性

    Belongs to the NAF1 family.

  • 細胞内局在

    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
      • hNAF1 antibody
      • Naf1 antibody
      • NAF1_HUMAN antibody
      • Nuclear assembly factor 1 homolog antibody
      • Nuclear assembly factor 1 ribonucleoprotein antibody
      see all

    画像

    • Western blot - Anti-NAF1 antibody [EPR11516] (ab171078)
      Western blot - Anti-NAF1 antibody [EPR11516] (ab171078)
      All lanes : Anti-NAF1 antibody [EPR11516] (ab171078) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Namalwa cell lysate
      Lane 5 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa

    • Flow Cytometry (Intracellular) - Anti-NAF1 antibody [EPR11516] (ab171078)
      Flow Cytometry (Intracellular) - Anti-NAF1 antibody [EPR11516] (ab171078)

      Intracellular flow cytometric analysis of permeabilized HeLa cells labeling NAF1 with ab171078 at 1/10 dilution (red),compared to a rabbit IgG negative control (green). 

       

       

    • Anti-NAF1 antibody [EPR11516] (ab171078)
      Anti-NAF1 antibody [EPR11516] (ab171078)

    参考文献 (0)

    ab171078 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab171078 は論文での使用が確認できていません。

    レビューと Q&A

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