AB208198

Anti-N6, N6-dimethyladenosine (m6,6A) 抗体 [EPR- 19831-44]

Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal N6, N6-dimethyladenosine (m6,6A) antibody. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 2 publications.

別名を表示する

Di-m6A, N6, N6 dimethyladenosine

2 Images

ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (AB208198)

ELISA

Lab

ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (AB208198)

Biotinylated m6,6A (modified), m6A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.

Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3'

Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m6A]*.mN.mN.mN.mN.mN 3'

Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

ELISA was performed on 1.0 μg/ml of antigen using ab208198 at a concentration range of 0.005-4.000 μg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (AB208198)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab208198 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR- 19831-44

アイソタイプ

IgG

キャリアフリー

アプリケーション

ELISA, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Has been developed to discriminate between the modified base N6, N6-dimethyladenosine (m6,6A) and the unmodified counterpart Adenosine (A).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "0.2 μg of ab208198 was coated onto 50 μL Dynabeads® sheep-anti-rabbit IgG.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

N6 N6-dimethyladenosine (m66A) also known as MMA is a modified nucleotide found in various RNA molecules. It possesses two methyl groups at the N6 position of the adenine base contributing to the stability and processing of RNA. The molecular mass of m66A is approximately 267.27 g/mol. This modification appears in a wide range of organisms from bacteria to humans indicating its importance across species. In human cells m66A is prevalent within the cytoplasm and nucleus where it plays a role in modulating RNA behavior.

Biological function summary

M66A can affect RNA's structural configuration and its interaction with other molecules. It is a part of the broader modification network within RNAs often found with other RNA modifications such as m6A and m1A. The presence of m66A influences RNA stability splicing and export which can alter the outcome of gene expression. In the context of gene expression regulation m66A interacts with multiple proteins that recognize and modify RNA structures. Consequently it forms part of the dynamic and responsive mechanisms that control cellular activities.

Pathways

M66A participates in the RNA metabolism and gene expression pathways. It associates with critical biological functions through interactions with proteins like METTL3 and FTO which regulate RNA methylation status. These pathways are important for processes such as translation regulation and RNA decay. METTL3 a methyltransferase adds methyl groups to RNA while FTO a demethylase removes them creating a balance in the modification states essential for proper cellular function.

M66A shows connections to several conditions including cancer and metabolic disorders. Changes in m66A levels might influence cellular transformation and tumor progression linking it to oncogenic processes. Specifically altered interactions with METTL3 have been observed in certain cancers due to its role in modifying RNA and affecting gene expression programs. Additionally m66A's involvement in metabolic regulation suggests a potential link to metabolic disorders wherein disruptions in RNA modifications may lead to impaired metabolism and related diseases.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information N6, N6-dimethyladenosine (m6,6A)

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Annals of medicine 57:2546670 PubMed40798940

2025

METTL3-mediated mA modification promotes intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Yang,Feihong Huang,Congyang Wang,Xiao Liang,Longao Huang,Hongyuan Xu,Jianwei Liu,Qingjun Wei,Hua Jiang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2417067 PubMed40019372

2025

ALKBH3-Mediated MA Demethylation of METTL3 Endows Pathological Fibrosis:Interplay Between MA and MA RNA Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Tu,Shuchen Gu,Ruoqing Xu,En Yang,Xin Huang,Hsin Liang,Shenying Luo,Haizhou Li,Yixuan Zhao,Tao Zan

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N6, N6-dimethyladenosine (m6,6A) 抗体 [EPR- 19831-44]

Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44]

ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (AB208198)

Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (AB208198)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Biochemicals

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise