Anti-N6-methyladenosine (m6A) 抗体 [17-3-4-1] (ab208577)
![Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)](https://www.abcam.co.jp/ps/products/208/ab208577/Images/ab208577-255281-anti-n6-methyladenosine-m6a-antibody-17-3-4-1-immunoprecipitation.jpg "Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)")
Key features and details
- Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A)
- Suitable for: Northwestern, IP, Southern Blot
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-N6-methyladenosine (m6A) antibody [17-3-4-1]
N6-methyladenosine (m6A) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A) -
由来種
Mouse -
特異性
This antibody recognises N6-methyladenosine in both modified RNA and DNA. -
アプリケーション
適用あり: Northwestern, IP, Southern Blotmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to bovine serum albumin. Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine
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ポジティブ･コントロール
- methylated RNA (negative control non methylated RNA); single stranded DNA;
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
17-3-4-1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab208577の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Northwestern |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
Best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.Used 3µg in 500µl for IP which yielded positive results. |
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| Southern Blot |
Use at an assay dependent concentration.
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| 特記事項 |
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Northwestern
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. Best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.Used 3µg in 500µl for IP which yielded positive results. |
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Southern Blot
Use at an assay dependent concentration. |
ターゲット情報
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関連性
N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. -
別名
- m6A antibody
- N6-methyladenosine antibody
画像
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Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)
N6-methyladenosine (m6A) was immunoprecipitated using ab208577 from radiolabeled synthetic methylated transcript (Bound); radiolabeled Non methylated control (Non bound) and radiolabeled Input RNA control.
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![Northwestern blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)](https://www.abcam.co.jp/ps/products/208/ab208577/Images/ab208577-255280-anti-n6-methyladenosine-m6a-antibody-17-3-4-1-northwestern-blot.jpg)
Northwestern blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)Use of ab208577 for northwestern blot using synthetic methlyated and non methlyated RNA.
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![Southern Blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)](https://www.abcam.co.jp/ps/products/208/ab208577/Images/ab208577-256037-anti-n6-methyladenosine-m6a-antibody-17-3-4-1-southern-blot.jpg)
Southern Blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)Lane 1 Single stranded DNA
Lane 2 Double stranded DNA
Labelled with ab208577. Arrow shows m6A containing fragment.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (62)
ab208577 は 62 報の論文で使用されています。
- Bao T et al. Recruitment of PVT1 Enhances YTHDC1-Mediated m6A Modification of IL-33 in Hyperoxia-Induced Lung Injury During Bronchopulmonary Dysplasia. Inflammation 47:469-482 (2024). PubMed: 37917328
- Wang J et al. WTAP enhances the instability of SYTL1 mRNA caused by YTHDF2 in bladder cancer. Histol Histopathol 39:633-646 (2024). PubMed: 37933909
- Liu Y et al. N6-methyladenosine-mediated overexpression of long noncoding RNA ADAMTS9-AS2 triggers neuroblastoma differentiation via regulating LIN28B/let-7/MYCN signaling. JCI Insight 8:N/A (2023). PubMed: 37991019
- Ling Y et al. Bisphenol A exposure induces testicular oxidative damage via FTO/m6A/Nrf2 axis during postnatal development in mice. J Appl Toxicol 43:694-705 (2023). PubMed: 36451259
- Das A et al. Identification of potential proteins translated from circular RNA splice variants. Eur J Cell Biol 102:151286 (2023). PubMed: 36645925